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Specifications
- Assay Type Sandwich ELISA
- Sample Types Serum, Plasma, Tissue Culture Media
- Sensitivity 30.5 pg/mL
- Species Human
- Assay Duration 3 Hours
- Samples/Plate 40 in Duplicate
- Readout Colorimetric, 450 nm
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Standard Curve
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Description
Assay Principle:
The ST2 Human ELISA Kit quantitatively measures Human ST2 levels in serum, plasma, and tissue culture media. The ST2 Human ELISA Kit is a sandwich ELISA with a run time of 3 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided ST2 standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our mouse anti-human ST2 and incubate covered shaking at room temperature for 1 hour. Add the Biotinylated human ST2 antibody, and continue to incubate covered at room temperature, shaking for 1 hour. Wash and add the Streptavidin-Peroxidase Conjugate and incubate the mixture covered, shaking at room temperature for 30 minutes. The immunological reaction occurs between the peroxidase-conjugated bound ST2 antibody and the ST2 antigen in the sample or standard.
After the 30-minute incubation, wash away the excess peroxidase-conjugated ST2 antibody and add the TMB substrate. The TMB substrate reacts with the bound peroxidase-conjugated ST2 antibody generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the ST2 concentration in the samples.
Background:
The ST2 protein, (also known as IL1RL1, DER4, T1, and FIT-1), is a Toll-like/IL-1-receptor superfamily member. The interleukin-1 (IL-1) receptor family has several members, including the classical interleukin-1 receptor (IL-1R) and the interleukin-18 receptor (IL-18R). In 1989, researchers identified ST2 as an orphan receptor. ST2 exerts functions in inflammatory processes like those within mast cells, type 2 CD4+ T-helper cells, and the production of Th2-associated cytokines. ST2 is a specific cellular marker that differentiates Th2 from Th1 T-cells. The gene for ST2 spans ~40 kb on human chromosome 2q12 and is part of the larger human IL-1 gene cluster of ~200 kb. ST2 is conserved across species, with homologs in mouse, rat, and fruitfly genomes. The ~37 kDa unglycosylated secreted protein is converted into a 60–70 kDa glycosylated product, the soluble form of ST2, sST2.