- Multi Species
- Multi-Format - Standard Range of 20-0.63 ng/mL or 2,000-63 pg/mL
- Sample - Serum, Plasma, Urine, Media and Dried Fecal Extracts
- Samples/Kit - 40 or 232 in Duplicate
- Sensitivity - Measure < 0.3 ng/mL in 10uL format
The DetectX® Thyroxine (T4) ELISA Kit is designed to quantitatively measure T4 present in serum, EDTA plasma, tissue culture media samples and fecal extracts. A thyroxine standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A thyroxine-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to T4 to each well. After an hour incubation the plate is washed and supplied substrate is added. The substrate reacts with the bound T4-peroxidase conjugate. After a 30 minute incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the T4 in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.