- Multi Species
- Sample - Urine, Media, and Extracted Serum, Plasma and Fecal Material
- Samples/Kit - 39 or 231 in Duplicate
- Sensitivity - Measure < 40 pg/mL
The DetectX® Triiodothyronine (T3) Enzyme Immunoassay kit is designed to quantitatively measure T3 present in urine, extracted serum and plasma, extracted dried fecal samples, and tissue culture media samples. This kit measures total T3 in extracted serum and plasma and in extracted fecal samples.
A T3 stock solution is provided to generate standard curves for the assay. Standards or diluted samples are pipetted into a coated microtiter plate. A T3-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep antibody to T3 to each well. After a two hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T3-peroxidase conjugate. After a 30 minute incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm.