Services

Under our direction, the high quality, chemical organic synthesis companies we work with make biological molecule derivatives suitable for effective conjugation.

We carry out protein labeling with the derivatives or peptides, utilizing porcine thyroglobulin (PTG) or BSA.

We prefer using PTG over KLH as a carrier protein because of its solubility and size.

For free thiol group containing derivatives, we pre-treat the PTG with a reagent to add maleimide groups.

For those derivatives with a free carboxylic acid, we chemically activate using DCC/NHS for coupling to protein lysine groups.

The PTG and BSA labeled antigens are purified on desalting columns, followed by analysis to estimate labeling efficiencies when possible.

Discovery
Tell us about the nature of the experiment, the number and types of samples, and any knowledge or past experiences you might have relevant to the work at hand.

Estimation and Proposal
Projects are carried out on a time and materials basis at a reasonable fee set after our discussion. We will provide a proposal for the work.

Cost Structure and Payments

You only pay for the Arbor Assays scientist’s time, plus any kits consumed, and special materials or reagents required to complete the work in question.

To initiate the project, a 50% deposit is needed to get started.

Kits
We prefer to use our kits for the analysis – this saves us time and you money. As all Arbor Assays kits are researched, developed, validated, manufactured and QC’d in-house we know everything about their performance.

If another company’s kit is required for analysis, we can work with that too.

Step 1: Test dilution series
To determine the best dilutions to run samples, we test a few with a dilution series.

Step 2: Assay data evaluation
The assay data is evaluated with a software program designed to give 4PLC fitting analysis and the resulting sample measurements.

Step 3: Data review

The data is critically reviewed for replicate precision and placement on the standard curve. Any samples with values outside of the standard range or with replicate precision not falling within the predefined range would be repeated.

Step 4: Reporting and Results
When sample testing is complete, a report is generated.

Step 5: Discussion
Discussion and any additional information needed can be provided.

Step 6: Final Payment
Final payment is only due once you’ve received your results.