- Multi Species
- USE - Measure Galactose Concentration
- SAMPLE - Serum, Plasma, Buffers and TCM
- SAMPLES/KIT - 88 in Duplicate
- STABILITY - All reagents stable at 4°C
The DetectX® Galactose Colorimetric Detection Kit is designed to quantitatively measure galactose in a variety of samples. A D-(+)-galactose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the Colorimetric Substrate and horseradish peroxidase and the reaction initiated by addition of galactose oxidase. The reaction is incubated at room temperature for 30 minutes. The galactose oxidase reacts with galactose to produce hydrogen peroxide which, in the presence of HRP, reacts with the colorless Colorimetric Substrate to produce a pink-colored product which is read at 560 nm. Increasing levels of galactose cause a linear increase in color.
Galactose is a hexose sugar that differs from glucose only by the configuration of the hydroxyl group at the carbon-4 position. Present as an anomeric mixture of α-D-galactose and ß-D-galactose, this monosaccharide exists abundantly in milk, dairy products and many other food types such as fruits and vegetables. Absorption of galactose in humans is mediated by the Na+/glucose co-transporters SGLT1 and SGLT2 from food across the brush border membrane of the proximal jejunum and renal epithelium. Other sources of galactose include endogenous production and natural turnover of glycolipids and glycoproteins. Adult humans can produce up to 2 grams of galactose per day.