- Most Sensitive
- Multi Species
- USE - Measure Thiol Content of Recombinant Proteins
- SAMPLE - Buffers
- SAMPLES/KIT - 39 in Duplicate
- SENSITIVE - Measure < 0.5 pmol Thiol/well
- STABILITY - Stable 4˚C Liquid Reagents
The DetectX® Thiol Fluorescent Detection kit allows users to accurately determine the extent of free thiol content in samples using a proprietary substrate, ThioStar®, that is converted to a brightly fluorescent product upon reaction with thiols in the sample. The fluorescent signal is read at 510nm with excitation at 410nm in a fluorescent plate reader. The thiol in the sample can either be one that is generated by a reaction, such as the end product of an enzymatic reaction that produces reduced glutathione, or can be the cysteine content of the protein to be measured. The graph shows the measurement of thiol content in a sample containing up to 4M guanidine HCl, equivalent to 8M GuHCl in the sample.
Free thiols in biological systems have important roles. Oxidatively modified thiol groups of cysteine residues are known to modulate the activity of a growing number of proteins. One of the most pressing problems with this approach is to accurately determine the extent of modification of specific amino acids, such as cysteine residues, in a complex protein sample.