Glutathione (GSH) Colorimetric Detection Kit

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Features

  • USE - Measure Glutathione in 20 MInutes or Kinetically
  • SAMPLE - Lysates, WB, RBCs, Serum, Plasma, Urine and Tissue
  • SAMPLES/KIT - 89 (Oxidized and Total) in Duplicate
  • STABILITY - Stable 4˚C Liquid Reagents

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Description

The DetectX® Glutathione (GSH) Colorimetric Detection kit utilizes a colorimetric substrate that reacts with the free thiol group on GSH to yield a highly colored product. All reagents are in solution and require simple dilution for use in the assay. Free GSH concentration in the sample is calculated from the difference between the total GSH determined and the GSH generated from GSSG. The concentration of GSH can be determined either as an endpoint read of the color developed at 405nm after 30 minutes or by measuring the rate of color development at 405nm.

Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios.

Glutathione Colorimetric Standard Curve

Data Reduction Template by MyAssays

Item Catalog # Price
Glutathione Colorimetric Detection Kit, 4 PlateK006-H1$285.00Inquire About This Product


Publications

Date Publication
Apr 11 2017 Restoring cytokine balance in HIV Positive Individuals with Low CD4 T Cell Counts
Feb 22 2017 Addition of exogenous enzymes to diets containing grape pomace: Effects on intestinal utilization of catechins and antioxidant status of chickens
Oct 07 2016 Glibenclamide impairs responses of neutrophils against Burkholderia pseudomallei by reduction of intracellular glutathione
Jul 26 2016 Study of Liposomal Reduced Glutathione effect on Immune Cell Function in HIV Positive Patients
Jul 18 2016 Crosstalk between cystine and glutathione is critical for the regulation of amino acid signaling pathways and ferroptosis
Jun 02 2016 Complement 3 Receptor Expression in Individuals with Type 2 Diabetes
May 29 2016 Analysis of Glutathione levels in the Brain tissue samples from HIV-1-Positive Individuals and subject with Alzheimer’s disease and its implication in the pathophysiology of the disease process
May 07 2016 Effect of dietary organic selenium on muscle proteolytic activity and water-holding capacity in pork
Feb 17 2016 Impact of simultaneous stimulation of 5-lipoxygenase and myeloperoxidase in human neutrophils
Feb 12 2016 Pitfalls in the analysis of the physiological antioxidant glutathione (GSH) and its disulfide (GSSG) in biological samples: An elephant in the room
Dec 10 2015 Silica Nanoparticles Induce Oxidative Stress and Autophagy but Not Apoptosis in the MRC-5 Cell Line
Oct 17 2015 Sex-Dependent effects of developmental arsenic exposure on methylation capacity and methylation regulation of the glucocorticoid receptor system in the embryonic mouse brain
Aug 25 2015 AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?
Jul 02 2015 Liposomal Glutathione Supplementation Restores TH1 Cytokine Response to Mycobacterium tuberculosis Infection in HIV-Infected Individuals
Jun 18 2015 Cigarette smoke-induced hypermethylation of the GCLC gene is associated with chronic obstructive pulmonary disease
Jun 09 2015 Magnetite nanoparticles induced adaptive mechanisms counteract cell death in human pulmonary fibroblasts
Jun 05 2015 Histopathology and oxidative stress analysis of concomitant misoprostol and celecoxib administration
May 29 2015 Histopatological alterations and oxidative stress in liver and kidney of Leuciscus cephalus following exposure to heavy metals in the Tur River, North Western Romania
Apr 01 2015 Hepatoprotective effects of a self-micro emulsifying drug delivery system containing Silybum marianum native seed oil against experimentally induced liver injury
Mar 19 2015 Investigating the Causes for Decreased Levels of Glutathione in Individuals with Type II Diabetes
Mar 01 2015 The effects of intraperitoneal administration of gold nanoparticles size and exposure duration on oxidative and antioxidants levels in various rat organs
Feb 24 2015 Cardiac Energy Dependence on Glucose Increases Metabolites Related to Glutathione and Activates Metabolic Genes Controlled by Mechanistic Target of Rapamycin
Nov 06 2014 Light-emitting diode therapy in exercise-trained mice increases muscle performance, cytochrome c oxidase activity, ATP and cell proliferation
Oct 27 2014 In vitro evaluation of inorganic mercury and methylmercury effects on the intestinal epithelium permeability
Apr 18 2014 The detrimental effects of acute hyperglycemia on myocardial glucose uptake
Nov 25 2013 Processing of glutathionylcobalamin by a bovine B12 trafficking chaperone bCblC involved in intracellular B12 metabolism
Oct 18 2013 Nitric oxide metabolites during anoxia and reoxygenation in the anoxia-tolerant vertebrate, Trachemys scripta
Aug 31 2013 Effect of GSTM1-Polymorphism on Disease Progression and Oxidative Stress in HIV Infection: Modulation by HIV/HCV Co-Infection and Alcohol Consumption
Jun 03 2013 Activating the Nrf2-mediated antioxidant response element restores barrier function in the alveolar epithelium of HIV-1 transgenic rats
Jun 01 2013 Water Soluble Pleurotus ostreatus Polysaccharide Down-Regulates the Expression of MMP-2 and MMP-9 in Caco-2 Cells
May 08 2013 Characterization of Dendritic Cell and Regulatory T Cell Functions against Mycobacterium tuberculosis Infection
Feb 14 2013 Glutathione Supplementation Improves Macrophage Functions in HIV
Jun 01 2012 Glutathione deficiency in type 2 diabetes impairs cytokine responses and control of intracellular bacteria.
Dec 29 2011 Unveiling the mechanisms for decreased glutathione in individuals with HIV infection.
Jul 16 2010 Loss of cystic fibrosis transmembrane conductance regulator function enhances activation of p38 and ERK MAPKs, increasing interleukin-6 synthesis in airway epithelial cells exposed to Pseudomonas aeruginosa.

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