Obelin (Recombinant) Photoprotein

Features

  • Most Sensitive
  • Multi Species
  • SUPERSENSITIVE - Measure < femtogram
  • FAST - 1 Second Read Time
  • CALCIUM DETECTION - Intra- and Intercellular Ca2+ Detection
  • OPTION WITH NO CALCIUM CHELATORS AVAILABLE

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Recombinant bioluminescent photoprotein from Obelia longissima. The photoprotein is approximately 21,000 molecular weight and contains the luminescent substrate, native coelenterazine, and oxygen bound to the protein. Upon addition of excess calcium ions, that fill the three calcium-binding sites, the oxygen reacts with the substrate to yield a rapid flash of blue light centered at 485 nm. This emission reaches a peak in less than 100msec. and decays in less than 1 second.

rec. Obelin Kinetics

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