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- Assay Type Competitive ELISA
- Sample Types Extracted Serum, Extracted Plasma (EDTA and Heparin), Urine, Saliva, Fecal Extracts, Tissue Culture Media
- Sensitivity 1.84 pg/mL
- Species Aldosterone is identical across species
- Assay Duration Overnight Assay (16-18 hours)
- Samples/Plate 39 in Duplicate
- Readout Chemiluminescent
- Standard Curve
The Aldosterone Chemiluminescent ELISA Kit quantitatively measures aldosterone in extracted serum, extracted plasma (EDTA and Heparin), urine, saliva, fecal extracts, and tissue culture media. The Aldosterone Chemiluminescent ELISA Kit is a competitive ELISA with a run time of 16 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided aldosterone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our donkey anti-sheep IgG antibody. Add the aldosterone peroxidase conjugate and the aldosterone polyclonal sheep antibody. Then incubate the mixture covered at 4°C, shaking overnight. The immunological reaction occurs between the anti-aldosterone antibody, the aldosterone antigen in the sample or standard, and the aldosterone-peroxidase conjugate. As the aldosterone concentration in the sample increases, the bound aldosterone-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the overnight incubation, wash away the excess aldosterone-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound aldosterone-peroxidase conjugate generating a chemiluminescent signal detected by a plate reader. Use the intensity and the standard curve to calculate the aldosterone concentration in the samples.
Aldosterone is a mineralocorticoid first isolated by Simpson and Tait’s husband and wife team at University College, London. Aldosterone controls the sodium-potassium balance through unidirectional salt reabsorption in various tissues and glands. It is synthesized from cholesterol in the zona glomerulosa of the adrenal cortex, and its secretion is regulated through the renin-angiotensin system. Angiotensin II and potassium stimulate primary secretion by increasing the steroid production rate. Peripheral aldosterone levels are dependent on age and body position. Aldosterone measurement is useful in investigating primary and secondary aldosteronism, including vascular disease, salt depletion, potassium loading, cardiac failure with ascites, and pregnancy.