Assay Principle: 

The Human B-Type Natriuretic Peptide (BNP) ELISA Kit quantitatively measures BNP in serum and EDTA plasma. This sandwich ELISA has a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay. Use our provided BNP standard to generate a standard curve for the assay.

Protocol Summary:

• Introduce the standards or diluted samples into the transparent microtiter plate coated with sheep anti-human B-Type Natriuretic Peptide antibody.
• Add the peroxidase-conjugated BNP monoclonal antibody to each well.
• Incubate the covered plate at room temperature with shaking for 2 hours, allowing the immunological reaction to occur.
• Following incubation, wash the plate to remove excess conjugated antibody. Add the TMB substrate, which interacts with the bound antibody to produce a detectable signal.
• Measure the absorbance at 450nm using a plate reader and use the standard curve to determine BNP concentrations.

Background:

B-Type Natriuretic Peptide (BNP), originally identified as brain natriuretic peptide, is a key hormone in the regulation of blood pressure and fluid balance in the body. While typically produced in the atrium of the heart under normal conditions, during heart failure BNP is synthesized and secreted by ventricular cardiomyocytes. This occurs as a response increased heart stretch, known as hypoxia. For this reason, BNP is particularly significant in cardiovascular diagnostics as elevated levels are strongly associated with heart failure and cardiac dysfunction. In clinical practice, a serum BNP concentration exceeding 100 pg/mL is commonly indicative of such conditions.

BNP also offers insights into the severity of some heart conditions. Research has shown that BNP levels correlate with the degree of left ventricular dysfunction and can predict outcomes in heart failure patients. Additionally, the peptide hormone has a role in counteracting the renin-angiotensin-aldosterone system, thereby reducing fluid overload and alleviating symptoms in heart failure.

The DetectX® Human B-Type Natriuretic Peptide (BNP) ELISA Kit is a valuable asset for cardiologic and clinical diagnostic research.