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Product Protocol Product SDS

Specifications

  • Assay Type Sandwich ELISA
  • Sample Types Serum, Plasma
  • Sensitivity 0.616 ng/mL
  • Species Human
  • Assay Duration 2.5 Hours
  • Samples/Plate 40 in Duplicate
  • Readout Colorimetric, 450 nm
  • Standard Curve C-Reactive Protein (CRP) Human ELISA Kit
  • Description

    Assay Principle: 

    The C-Reactive Protein (CRP) Human ELISA Kit quantitatively measures CRP levels in serum and plasma. The C-Reactive Protein (CRP) Human ELISA Kit is a sandwich ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

    Use our provided CRP standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our mouse anti-human CRP. Add the peroxidase-conjugated human CRP antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the peroxidase-conjugated human CRP antibody and the CRP antigen in the sample or standard. 

    After the 2-hour incubation, wash away the excess peroxidase-conjugated human CRP antibody and add the TMB substrate. The TMB substrate reacts with the bound peroxidase-conjugated CRP antibody generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the CRP concentration in the samples.

    Background:

    C-Reactive Protein (CRP) is a ring-shaped, pentameric serum protein produced by the liver. As an acute phase inflammation marker released in response to IL-6 signaling by T-cells, macrophages, and adipocytes, CRP levels rise in a wide range of acute and chronic inflammatory conditions. These include infection, inflammatory disease, tissue injury, cardiovascular disease, and malignancy. Monitoring CRP levels is often used to assess inflammatory levels in the body. High CRP levels indicate inflammation either from an acute infection or chronic inflammation. Chronically elevated CRP indicates cardiovascular diseases and is a significant risk factor for atherosclerosis.

    In conjugation with Myeloperoxidase, CRP assessment has been used clinically to accurately predict mortality risk for cardiac patients.