• Assay Type Sandwich ELISA
  • Sample Types Serum, Plasma, Tissue Culture Media
  • Sensitivity 0.579 pg/mL
  • Species Human, Bovine, Porcine, Dog, Rat, Mouse
  • Assay Duration 1.5 Hours
  • Samples/Plate 39 in Duplicate
  • Readout Colorimetric, 450 nm
  • Standard Curve
  • Description

    Endothelin-1 (ET-1), a peptide of 21 amino acid residues, is a pleiotropic molecule known for its action as a potent vasoconstrictor. ET-1 is one of a family of three proteins encoded by distinct genes. All members of the Endothelin family contain two essential disulfide bridges and six conserved amino acid residues at the C-terminus. Human ET-1 is initially synthesized as a pre-pro-polypeptide of 212 amino acids. It is proteolytically cleaved by a signal peptidase to produce pro-ET-1 and further processed by a Furin-like protease to yield Big ET-1. The vascular endothelium is an abundant source of ET-1. It may also be expressed by leukocytes, smooth muscle cells, mesangial cells, cardiac myocytes, and astrocytes. ET-1 can be induced in endothelial cells by many factors including mechanical stimulation, various hormones, and pro-inflammatory cytokines. Production is inhibited by nitric oxide (NO), cyclic nucleotides, prostacyclin, and atrial natriuretic peptide (ANP). With this kit you can quantitatively measure Et-1.