Specifications

  • Catalog Number K043-H
  • Assay Type Detection Kit
  • Sample Types Serum, Plasma (EDTA and Heparin), Urine, Cell Lysates, Tea, Fruit Juice, Beer, Cider, Cosmetics, Food Extracts
  • Sensitivity 8.06 µM
  • Species Species Independent
  • Assay Duration 30 Minutes
  • Samples/Plate 41 in Duplicate
  • Readout Colorimetric, 560 nm

Images

Assay Principle: 

The FRAP™ (Ferric Reducing Antioxidant Power) Detection Kit quantitatively measures antioxidant status in serum, plasma (EDTA and Heparin), urine, cell lysates, tea, fruit juice, beer, cider, cosmetics, and food extracts. The kit, with a convenient 30-minute run time, allows for a comprehensive assessment of antioxidant capacity. Users should refer to the complete kit insert for detailed instructions before conducting the assay. A Ferrous Chloride Standard is included in the FRAP™ Detection Kit for generating an accurate standard curve.

Protocol Summary:

  • Introduce standards or diluted samples into the provided transparent microtiter plate.
  • Add FRAP Color Solution to each well, ensuring thorough mixing.
  • Incubate the mixture at room temperature for 30 minutes. During incubation, the FRAP Color Solution reacts with antioxidants in the sample, producing a blue-colored product.
  • After incubation, use a plate reader to detect the signal at 560nm. Calculate antioxidant status using the intensity data and standard curve.

Background:

The balance between reactive oxygen species (ROS) production and antioxidant defenses is important in maintaining cellular health. ROS, including free radicals, are generated as by-products of normal metabolism and can cause significant damage to biological systems if not adequately controlled. Antioxidants neutralize these free radicals, preventing potential harm.

Research has linked excessive free radical activity to various severe diseases, such as Parkinson’s and Alzheimer’s Disease, atherosclerosis, heart attacks, and chronic fatigue syndrome. However, some level of controlled oxidative stress can be beneficial, triggering processes like Mitohormesis that may prevent aging.

Given the prevalence of oxidative stress in a significant portion of the population (65 – 70%), the DetectX® FRAP™ Detection Kit is an important tool for researchers and healthcare professionals. It provides insights into the antioxidant status of biological samples and products, aiding in the study of diseases related to oxidative stress, the effectiveness of antioxidant-rich foods and beverages, and the development of cosmetic and pharmaceutical products.

FRAP™ (Ferric Reducing Antioxidant Power) Detection Kit

$604.00

In Stock

The DetectX® FRAP™ (Ferric Reducing Antioxidant Power) Detection Kit quantitatively measures antioxidant status in a variety of samples.

SKU: K043-H1 Category:

Research Areas