FRAP™ (Ferric Reducing Antioxidant Power) Detection Kit

Research Areas

Documentation

Specifications

• Assay Type Detection Kit
• Sample Types Serum, Plasma (EDTA and Heparin), Urine, Cell Lysates, Tea, Fruit Juice, Beer, Cider, Cosmetics, Food Extracts
• Sensitivity 8.06 µM
• Species Species Independent
• Assay Duration 30 Minutes
• Samples/Plate 41 in Duplicate
• Readout Colorimetric, 560 nm
• Standard Curve
• Description

  Assay Principle: 

  The FRAP™ (Ferric Reducing Antioxidant Power) Detection Kit quantitatively measures antioxidant status in serum, plasma (EDTA and Heparin), urine, cell lysates, tea, fruit juice, beer, cider, cosmetics, and food extracts. The FRAP™ (Ferric Reducing Antioxidant Power) Detection Kit is a Detection Kit with a run time of 30 minutes. Please read the complete kit insert for more information before performing this assay.

  Use our provided Ferrous Chloride Standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate. Add the FRAP Color Solution to each well, tapping the plate to ensure sufficient mixing of reagents. Then, incubate the mixture at room temperature for 30 minutes. The color-generating reaction occurs when FRAP Color Solution reacts with antioxidant species in the standard or sample to produce a blue-colored product. 

  After the 30-minute incubation, use a plate reader to detect and record the generated signal at 560nm. Use the intensity and the standard curve to calculate the antioxidant status in the samples.

  Background:

  Normal aerobic metabolism produces potentially harmful reactive oxygen species (ROS). ‘‘Free Radicals’’ (FR) are usually removed or inactivated in vivo by a team of antioxidants. They are chemically stable atoms and molecules with one or more free electrons. Reactive free radicals can attack almost all biomolecules. Free radicals are responsible for many pathological processes, or they can be generated from the pathological stage and cause significant secondary damage to biological systems and cells. 

  Connections between free radicals and some severe diseases, including Parkinson’s and Alzheimer’s Disease, atherosclerosis, heart attacks, and chronic fatigue syndrome, have been demonstrated. However, short-term oxidative stress, the unbalance between the formation and scavenging of the reactive oxygen species, may be critical in preventing aging due to the triggering of the process known as Mitohormesis. On average, oxidative stress caused by free radicals impacts 65 – 70% of the population.