Glutathione (GSH) Colorimetric Detection Kit


  • Multi Species
  • Use - Measure Glutathione in 20 MInutes or Kinetically
  • Sample - Lysates, WB, RBCs, Serum, Plasma, Urine and Tissue
  • Samples/Kit - 89 (Oxidized and Total) in Duplicate
  • Stability - Stable 4˚C Liquid Reagents

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The DetectX® Glutathione (GSH) Colorimetric Detection Kit utilizes a colorimetric substrate that reacts with the free thiol group on GSH to yield a highly colored product. All reagents are in solution and require simple dilution for use in the assay. Free GSH concentration in the sample is calculated from the difference between the total GSH determined and the GSH generated from GSSG. The concentration of GSH can be determined either as an endpoint read of the color developed at 405nm after 30 minutes or by measuring the rate of color development at 405nm.

Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios.

Glutathione Colorimetric Standard Curve

Data Reduction Template by MyAssays

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+ Publications 58 total publications

Clinical trials of TB-HDT candidates

Dec 01 2020 | (SPECIES: Human, SAMPLE: Blood)

Pantoea ananatis defeats Allium chemical defenses with a plasmid-borne virulence gene cluster

Feb 13 2020 | (SPECIES: Plant (Onion, Garlic), SAMPLE: Plasmid)

The detrimental effects of acute hyperglycemia on myocardial glucose uptake

Apr 18 2014 | (SPECIES: Rat, SAMPLE: Cell Culture)

Nitric oxide metabolites during anoxia and reoxygenation in the anoxia-tolerant vertebrate, Trachemys scripta

Oct 18 2013 | (SPECIES: Turtle (Red-Eared Slider), SAMPLE: Plasma, Heart Tissue, Liver Tissue)

Glutathione Supplementation Improves Macrophage Functions in HIV

Feb 14 2013 | (SPECIES: Human, SAMPLE: Blood)

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