Glutathione (GSH) Colorimetric Detection Kit

Research Areas

Documentation

Specifications

• Assay Type Detection Kit
• Sample Types Whole Blood, Serum, Plasma (EDTA and Heparin), Erythrocytes, Urine, Cell Lysates, Tissue
• Sensitivity 0.634 μM
• Species GSH is identical across species
• Samples/Plate 41 in Duplicate
• Assay Duration 20 Minutes
• Readout Colorimetric, 405 nm
• Standard Curve
• Description

  Assay Principle: 

  The Glutathione (GSH) Colorimetric Detection Kit quantitatively measures GSH levels in whole blood, serum, plasma (EDTA and Heparin), erythrocytes, urine, cell lysates, and tissue. The Glutathione (GSH) Colorimetric Detection Kit is a Detection Kit with a run time of 20 minutes. Please read the complete kit insert for more information before performing this assay.

  Use our provided Glutathione standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a clear microtiter plate. Add Detection Reagent to each well, tapping the plate to ensure sufficient mixing of reagents. Then incubate the mixture covered at room temperature for 20 minutes. The color-generating reaction occurs between the Detection Reagent and the GSH within the sample or standard. 

  After the 20-minute incubation, use a plate reader to detect the generated signal at 405nm. Use the intensity and the standard curve to calculate the GSH concentration in the samples.

  Background:

  Glutathione (L-γ-glutamyl-L-cysteinyl glycine; GSH) is the highest concentration of non-protein thiol in mammalian cells and is present in concentrations of 0.5 – 10 mM. GSH is a tripeptide molecule. It contains a peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. It is an antioxidant, preventing damage to important cellular components caused by reactive oxygen species. Enzymes convert Glutathione to its oxidized form, glutathione disulfide (GSSG). By serving as an electron donor, Glutathione reduces disulfide bonds formed within cytoplasmic proteins to cysteines. Its reduced form is most common since glutathione reductase is constitutive and inducible upon oxidative stress. Investigators use the ratio of reduced Glutathione to oxidized Glutathione within cells to measure cellular toxicity.

• Structure