Research Areas



  • Assay Type Sandwich ELISA
  • Sample Types Serum, Plasma (EDTA and Heparin), Urine, Tissue Culture Media
  • Sensitivity 58 pg/mL
  • Species Human
  • Assay Duration 2 Hours
  • Samples/Plate 40 in Duplicate
  • Readout Colorimetric, 450 nm
  • Standard Curve
  • Description

    Assay Principle: 

    The Cystatin C Human ELISA Kit quantitatively measures Human Cystatin C levels in serum, plasma (EDTA and Heparin), urine, and tissue culture media. The Cystatin C Human ELISA Kit is a sandwich ELISA with a run time of 2 hours. Please read the complete kit insert for more information before performing this assay.

    Use our provided human Cystatin C standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our mouse anti-human Cystatin C antibody and incubate at room temperature for 1 hour. Add the peroxidase-conjugated human Cystatin C monoclonal antibody. Then incubate the mixture covered at room temperature for 30 minutes. The immunological reaction occurs between the peroxidase-conjugated BNP monoclonal antibody and the BNP antigen in the sample or standard. 

    After the 30-minute incubation, wash away the excess peroxidase-conjugated Cystatin C monoclonal antibody and add the TMB substrate. The TMB substrate reacts with the bound peroxidase-conjugated Cystatin C monoclonal antibody generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the Cystatin C concentration in the samples.


    Cystatin C is a low molecular weight non-glycosylated protein (13 kDa) in the cystatin superfamily. Cystatin C belongs to the cysteine proteinase inhibitor group. The imbalance between Cystatin C and cysteine proteinases is associated with conditions such as cancer, Alzheimer’s disease, multiple sclerosis, and hereditary Cystatin C amyloid angiopathy.

    Glomerular filtration in the kidneys removes Cystatin C from the blood plasma. The proximal tubular cells then reabsorb Cystatin C and degrade it. A linear relationship exists between the reciprocal Cystatin C concentration in plasma and the glomerular filtration rate (GFR). Cystatin C serum concentration is not affected by age, gender, or body mass, suggesting it may be a better marker for GFR than serum creatinine.