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Specifications
- Assay Type Sandwich ELISA
- Sample Types Serum, Plasma (EDTA and Heparin), Urine, Tissue Culture Media
- Sensitivity 58 pg/mL
- Species Human
- Assay Duration 2 Hours
- Samples/Plate 40 in Duplicate
- Readout Colorimetric, 450 nm
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Standard Curve
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Description
Assay Principle:
The Cystatin C Human ELISA Kit quantitatively measures Human Cystatin C levels in serum, plasma (EDTA and Heparin), urine, and tissue culture media. The Cystatin C Human ELISA Kit is a sandwich ELISA with a run time of 2 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided human Cystatin C standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our mouse anti-human Cystatin C antibody and incubate at room temperature for 1 hour. Add the peroxidase-conjugated human Cystatin C monoclonal antibody. Then incubate the mixture covered at room temperature for 30 minutes. The immunological reaction occurs between the peroxidase-conjugated BNP monoclonal antibody and the BNP antigen in the sample or standard.
After the 30-minute incubation, wash away the excess peroxidase-conjugated Cystatin C monoclonal antibody and add the TMB substrate. The TMB substrate reacts with the bound peroxidase-conjugated Cystatin C monoclonal antibody generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the Cystatin C concentration in the samples.
Background:
Cystatin C is a non-glycosylated protein of low molecular weight (13 kDa) in the cystatin superfamily. Cystatin C belongs to the cysteine proteinase inhibitor group and is associated with several pathological states. The imbalance between Cystatin C and cysteine proteinases is associated with conditions such as cancer, Alzheimer’s disease, multiple sclerosis, and hereditary Cystatin C amyloid angiopathy. Altered Cystatin C levels are associated with myocardial infarction, coronary death, and angina pectoris.
Cystatin C is removed from blood plasma by glomerular filtration in the kidneys. It is reabsorbed by the proximal tubular cells and degraded. A linear relationship exists between the reciprocal Cystatin C concentration in plasma and the glomerular filtration rate (GFR). Cystatin C serum concentration is not affected by age, gender, or body mass, suggesting it may be a better marker for GFR than serum creatinine.