Cystatin C Human ELISA Kit

Assay Principle:

The DetectX® Cystatin C Human ELISA Kit accurately measures human Cystatin C levels in serum, plasma (EDTA and Heparin), urine, and tissue culture media. This sandwich ELISA offers a run time of 2 hours with straightforward and efficient methodology. For comprehensive guidance on performing the assay, consult the detailed instructions in the kit insert. The kit includes a human Cystatin C standard to establish a precise standard curve.

Protocol Summary:

• Add standards or diluted samples to the provided transparent microtiter plate coated with mouse anti-human Cystatin C antibody. Incubate at room temperature for 1 hour.
• Introduce the peroxidase-conjugated human Cystatin C monoclonal antibody and incubate for an additional 30 minutes.
• Following incubation, wash away excess antibody and add the TMB substrate. The substrate reacts with the bound antibody, creating a measurable signal.
• Use a plate reader to detect the signal at 450nm and calculate the Cystatin C concentration based on the standard curve.

Background:

Cystatin C is a low molecular weight non-glycosylated protein (13 kDa) in the cystatin superfamily. Cystatin C belongs to the cysteine proteinase inhibitor group. The imbalance between Cystatin C and cysteine proteinases is associated with conditions such as cancer, Alzheimer’s disease, multiple sclerosis, and hereditary Cystatin C amyloid angiopathy.

Glomerular filtration in the kidneys removes Cystatin C from the blood plasma. The proximal tubular cells then reabsorb Cystatin C and degrade it. A linear relationship exists between the reciprocal Cystatin C concentration in plasma and the glomerular filtration rate (GFR). Cystatin C serum concentration is not affected by age, gender, or body mass, suggesting it may be a better marker for GFR than serum creatinine.

The DetectX® Cystatin C Human ELISA Kit is an essential tool for research and clinical studies focusing on kidney function, as well as investigations into the pathogenesis and progression of diseases where Cystatin C plays a significant role.