• Catalog Number K012-H
  • Assay Type Sandwich ELISA
  • Sample Types Serum, Plasma (EDTA and Heparin), Urine, Tissue Culture Media
  • Sensitivity 58 pg/mL
  • Species Human
  • Assay Duration 2 Hours
  • Samples/Plate 40 in Duplicate
  • Readout Colorimetric, 450 nm


Assay Principle:

The DetectX® Cystatin C Human ELISA Kit accurately measures human Cystatin C levels in serum, plasma (EDTA and Heparin), urine, and tissue culture media. This sandwich ELISA offers a run time of 2 hours with straightforward and efficient methodology. For comprehensive guidance on performing the assay, consult the detailed instructions in the kit insert. The kit includes a human Cystatin C standard to establish a precise standard curve.

Protocol Summary:

  • Add standards or diluted samples to the provided transparent microtiter plate coated with mouse anti-human Cystatin C antibody. Incubate at room temperature for 1 hour.
  • Introduce the peroxidase-conjugated human Cystatin C monoclonal antibody and incubate for an additional 30 minutes.
  • Following incubation, wash away excess antibody and add the TMB substrate. The substrate reacts with the bound antibody, creating a measurable signal.
  • Use a plate reader to detect the signal at 450nm and calculate the Cystatin C concentration based on the standard curve.


Cystatin C is a low molecular weight non-glycosylated protein (13 kDa) in the cystatin superfamily. Cystatin C belongs to the cysteine proteinase inhibitor group. The imbalance between Cystatin C and cysteine proteinases is associated with conditions such as cancer, Alzheimer’s disease, multiple sclerosis, and hereditary Cystatin C amyloid angiopathy.

Glomerular filtration in the kidneys removes Cystatin C from the blood plasma. The proximal tubular cells then reabsorb Cystatin C and degrade it. A linear relationship exists between the reciprocal Cystatin C concentration in plasma and the glomerular filtration rate (GFR). Cystatin C serum concentration is not affected by age, gender, or body mass, suggesting it may be a better marker for GFR than serum creatinine.

The DetectX® Cystatin C Human ELISA Kit is an essential tool for research and clinical studies focusing on kidney function, as well as investigations into the pathogenesis and progression of diseases where Cystatin C plays a significant role.

Cystatin C Human ELISA Kit


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The DetectX Cystatin C ELISA Kit is designed to measure Human Cystatin C levels in a variety of biological matrices

SKU: K012-H1 Category:

Research Areas