Corticosterone Chemiluminescent ELISA Kits

Research Areas

Documentation

Specifications

• Assay Type Competitive ELISA
• Sample Types Serum, Plasma, Urine, Respiratory Vapor, Tissue Culture Media, Fecal Extracts, Feathers, Hair
• Sensitivity 6.71 pg/mL
• Species Corticosterone is identical across species
• Assay Duration 2 Hours
• Samples/Plate 39 in Duplicate
• Readout Chemiluminescent
• Standard Curve
• Description

  Assay Principle: 

  The Corticosterone Chemiluminescent ELISA Kits quantitatively measure corticosterone in Serum, Plasma, Urine, Respiratory Vapor, Fecal Extracts, Feathers, Hair, and Tissue Culture Media. The Corticosterone Chemiluminescent ELISA Kits are a competitive ELISA. Please read the complete kit insert for more information before performing this assay.

  Use our provided corticosterone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our donkey anti-sheep IgG antibody. Add the corticosterone peroxidase conjugate and the corticosterone polyclonal sheep antibody. Then incubate the mixture for 2 hours, shaking at room temperature. The immunological reaction occurs between the anti-corticosterone polyclonal antibody, the corticosterone antigen in the sample or standard, and the corticosterone-peroxidase conjugate. As the corticosterone concentration in the sample increases, the bound corticosterone-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

  After the 2-hour incubation, wash away the excess corticosterone-peroxidase and add the chemiluminescent substrate. The chemiluminescent substrate reacts with the bound corticosterone-peroxidase conjugate generating a light detected by a plate reader. Use the intensity and the standard curve to calculate the corticosterone concentration in the samples. 

  Background: 

  Corticosterone (Kendall’s Compound ‘B’) is a glucocorticoid secreted by the cortex of the adrenal gland. It is produced in the adrenal cortex upon stimulation by ACTH and is the precursor of aldosterone. Corticosterone is a significant indicator of stress and is the prominent stress steroid produced in non-human mammals. Studies involving corticosterone and stress levels have found evidence of impairment of long-term memory retrieval, chronic corticosterone elevation due to dietary restrictions, and corticosterone elevation in response to burn injuries. In addition to stress levels, corticosterone plays a decisive role in sleep-wake patterns. 

• Structure