Corticosterone ELISA Kit


  • Multi Species
  • Use - Measure Corticosterone in as little as 2μL sample
  • Sample - Serum, Plasma, Urine, Saliva, TCM and Extracted Fecal, Hair, Feather, Nail, Baleen, Respiratory Vapor or Mucus Samples
  • Samples/Kit - 38 or 230 in Duplicate
  • Stability - Liquid 4˚C Stable Reagents

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The DetectX® Corticosterone Enzyme Immunoassay Kit measures corticosterone present in tissue culture, serum, plasma and fecal samples. The kit has been developed for extracted fecal samples and has been validated with multiple animal species. Diluted samples are pipetted into a clear microtiter plate coated with an antibody. A corticosterone-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is read at 450nm.

Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. Corticosterone is a major indicator of stress and is the major stress steroid produced in non-human mammals. Studies involving corticosterone and levels of stress include impairment of long term memory retrieval, chronic corticosterone elevation due to dietary restrictions and in response to burn injuries. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns.


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+ Publications 226 total publications

Subchronic stress effects on vascular reactivity in C57BL/6 strain mice

Mar 09 2019 | (SPECIES: Mouse, SAMPLE: Plasma)

E47 modulates hepatic glucocorticoid action

Jan 18 2019 | (SPECIES: Mouse, SAMPLE: Plasma)

Age, seasonality, and correlates of aggression in female Apennine chamois

Oct 02 2018 | (SPECIES: Bovoid, SAMPLE: Plasma)

Mouse models of maternal immune activation: Mind your caging system!

Jul 17 2018 | (SPECIES: Mouse, SAMPLE: Plasma)

An evaluation of methods for measuring stress in broiler chickens

Jun 13 2018 | (SPECIES: Chicken, SAMPLE: Feather)

Neuromodulation Device

Nov 23 2017 | (SPECIES: Rat, SAMPLE: Plasma)

Causes and consequences of life- history variation

Nov 01 2017 | (SPECIES: Bird, SAMPLE: Feather)

Chronic noise exposure in the spontaneously hypertensive rat

Sep 21 2017 | (SPECIES: Rat, SAMPLE: Plasma)

Corticosterone regulation in house sparrows invading Senegal

May 27 2017 | (SPECIES: Bird, SAMPLE: Plasma)

CRF1 receptor-deficiency increases cocaine reward

Jan 27 2017 | (SPECIES: Mouse, SAMPLE: Plasma)


Aug 16 2016 | (SPECES: Mouse, SAMPLE: Serum)

Characterizing predictors of survival in the American pika (Ochotona princeps)

Jun 17 2016 | (SPECIES: American Pika, SAMPLE: Plasma & Fecal)

Hypothalamic CRH neurons orchestrate complex behaviours after stress

Jun 16 2016 | (SPECIES: Mouse, SAMPLE: Blood)

Chronic Stress and RCAN1 Expression

Apr 05 2016 | (SPECIES: Mouse, SAMPLE: Fecal)

Welfare indicators in laying hens in relation to nest exclusion

Mar 18 2016 | (SPECIES: Bird, SAMPLE: Fecal)

In vivo imaging of CREB phosphorylation in awake-mouse brain

Jun 05 2015 | (SPECIES: Mouse, SAMPLE: Serum)

Does sleep deprivation lower corticosterone levels in mice?

May 29 2015 | (SPECIES: Mouse, SAMPLE: Serum)

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