Corticosterone Multi-Format ELISA Kits

Research Areas

Documentation

Specifications

• Assay Type Competitive ELISA
• Sample Types Serum, Plasma, Urine, Respiratory Vapor, Tissue Culture Media, Feather, Hair, Fecal Extracts
• Sensitivity 20.9 pg/mL in 50 µL format, 14.4 pg/mL in 100µL format
• Species Corticosterone is identical across species
• Assay Duration 1.5 Hours
• Samples/Plate 37 in Duplicate
• Readout Colorimetric, 450 nm
• Standard Curve
• Description

  Assay Principle: 

  The Corticosterone Multi-Format ELISA Kit quantitatively measures Corticosterone in serum, plasma, urine, respiratory vapor, tissue culture media, feather, hair, and fecal extracts. The Corticosterone Multi-Format ELISA Kit is a competitive ELISA with a run time of 1.5 hours. This Multi-Format kit allows you to utilize varying sample starting amounts depending on the sensitivity anticipated for the assay. Use 50 μL of input for an expected sensitivity of 20.9 pg/mL and 100 μL for a sensitivity of 14.4 pg/mL. Please read the complete kit insert for more information before performing this assay.

  Use our provided corticosterone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our donkey anti-sheep IgG antibody. Add the corticosterone peroxidase conjugate and the corticosterone polyclonal sheep antibody. Then incubate the mixture for 1 hour, shaking at room temperature. The immunological reaction occurs between the anti-corticosterone polyclonal antibody, the corticosterone antigen in the sample or standard, and the corticosterone-peroxidase conjugate. As the corticosterone concentration in the sample increases, the bound corticosterone-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

  After the 1-hour incubation, wash away the excess corticosterone-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound corticosterone-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the corticosterone concentration in the samples.

  Background:

  Corticosterone (Kendall’s Compound’ B’) is a steroid hormone secreted by the cortex of the adrenal gland. The adrenal cortex produces this Aldosterone precursor, Corticosterone, upon ACTH stimulation. Corticosterone (CORT) is a significant indicator of stress and is the prominent stress steroid produced in non-human mammals.

  Acute levels of CORT are observed in the “fight-or-flight” response, while chronic levels are associated with serious health concerns. CORT is a memory-modulating hormone that can cross the blood-brain barrier to interact with multiple brain regions and influence memory consolidation. Severe burn injuries and drastic dietary restrictions induce elevated Corticosterone levels. In addition to stress levels, Corticosterone plays a decisive role in sleep-wake patterns via its alertness-supporting effects, increasing wakefulness. Corticosterone also plays significant developmental and behavioral functions in birds.

• Structure