Corticosterone ELISA Kit


  • Most Sensitive
  • Multi Species
  • Multi-Format - Standard Range of 10,000 - 39.06 pg/mL or 5,000 - 19.53 pg/mL
  • Use – Measure Corticosterone in as little as 2 μL sample
  • Sample – Serum, Plasma, Saliva, Urine, Fecal Extracts, and Tissue Culture Media
  • Samples/Kit – 37 or 229 in Duplicate
  • Sensitivity – 20.9 pg/mL in 50 μL format, 17.5 pg/mL in 100 μL format

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The DetectX® Corticosterone Enzyme Immunoassay Kit measures corticosterone present in serum, plasma, saliva, urine, extracted fecal samples, and tissue culture media. The kit has been developed for extracted fecal samples and has been validated with multiple animal species. Diluted samples are pipetted into a clear microtiter plate coated with an antibody. A corticosterone-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is read at 450nm.

Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. Corticosterone is a major indicator of stress and is the major stress steroid produced in non-human mammals. Studies involving corticosterone and levels of stress include impairment of long term memory retrieval, chronic corticosterone elevation due to dietary restrictions and in response to burn injuries. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns.

Calculation of Results

Online tools from MyAssays to calculate the data:

50 μL Assay Format

100 μL Assay Format

View All Publications

+ Publications 373 total publications

Seasonal changes in steroid and thyroid hormone content in shed skins of the tegu lizard Salvator merianea

Aug 11 2021 | (SPECIES: Lizard (Tegu Lizard), SAMPLE: Plasma, Sheds)

Investigation of keratinase digestion to improve steroid hormone extraction from diverse keratinous tissues

Apr 20 2021 | (SPECIES: Whale, Tefu Lizard, Ground Squirrel, Purple Martins, Short-beaked Echidna, SAMPLE: Baleen, Skin, Hair, Spine)

Effects of feather processing methods on quantity of extracted corticosterone in broiler chickens

Feb 01 2021 | (SPECIES: Bird, Chicken, SAMPLE: Feather Extract)

Timing of food intake drives the circadian rhythm of blood pressure

Nov 24 2020 | (SPECIES: Mouse, SAMPLE: Plasma)

Effects of temperature on plasma corticosterone in a native lizard

Oct 01 2020 | (SPECIES: Fence Lizard, SAMPLE: Plasma)

Exogenous flupirtine as potential treatment for CLN3 disease

Aug 11 2020 | (SPECIES: Mouse, SAMPLE: Serum)

Mental stress promotes the proliferation of endometriotic lesions in mice

Aug 06 2020 | (SPECIES: Mouse, SAMPLE: Plasma)

Methods and compositions for the treatment of obesity

Jun 25 2020 | (SPECIES: Mouse, SAMPLE: Serum)

Social Stress Is Lethal in the mdx Model of Duchenne Muscular Dystrophy

Mar 16 2020 | (SPECIES: Mouse, SAMPLE: Urine)

Immune-Endocrine Links to Gregariousness in Wild House Mice

Feb 05 2020 | (SPECIES: Mouse, SAMPLE: Serum)

Altitudinal Effects on Innate Immune Response of a Subterranean Rodent

Jan 24 2020 | (SPECIES: Molerat, SAMPLE: Serum)

Late-onset renal hypertrophy and dysfunction in mice lacking CTRP1

Dec 05 2019 | (SPECIES: Mouse, SAMPLE: Serum)

Subchronic stress effects on vascular reactivity in C57BL/6 strain mice

Mar 09 2019 | (SPECIES: Mouse, SAMPLE: Plasma)

E47 modulates hepatic glucocorticoid action

Jan 18 2019 | (SPECIES: Mouse, SAMPLE: Plasma)

Age, seasonality, and correlates of aggression in female Apennine chamois

Oct 02 2018 | (SPECIES: Bovoid, SAMPLE: Plasma)

Mouse models of maternal immune activation: Mind your caging system!

Jul 17 2018 | (SPECIES: Mouse, SAMPLE: Plasma)

An evaluation of methods for measuring stress in broiler chickens

Jun 13 2018 | (SPECIES: Chicken, SAMPLE: Feather)

Neuromodulation Device

Nov 23 2017 | (SPECIES: Rat, SAMPLE: Plasma)

Causes and consequences of life- history variation

Nov 01 2017 | (SPECIES: Bird, SAMPLE: Feather)

Chronic noise exposure in the spontaneously hypertensive rat

Sep 21 2017 | (SPECIES: Rat, SAMPLE: Plasma)

Corticosterone regulation in house sparrows invading Senegal

May 27 2017 | (SPECIES: Bird, SAMPLE: Plasma)

CRF1 receptor-deficiency increases cocaine reward

Jan 27 2017 | (SPECIES: Mouse, SAMPLE: Plasma)


Aug 16 2016 | (SPECES: Mouse, SAMPLE: Serum)

Characterizing predictors of survival in the American pika (Ochotona princeps)

Jun 17 2016 | (SPECIES: American Pika, SAMPLE: Plasma & Fecal)

Hypothalamic CRH neurons orchestrate complex behaviours after stress

Jun 16 2016 | (SPECIES: Mouse, SAMPLE: Blood)

Chronic Stress and RCAN1 Expression

Apr 05 2016 | (SPECIES: Mouse, SAMPLE: Fecal)

Welfare indicators in laying hens in relation to nest exclusion

Mar 18 2016 | (SPECIES: Bird, SAMPLE: Fecal)

In vivo imaging of CREB phosphorylation in awake-mouse brain

Jun 05 2015 | (SPECIES: Mouse, SAMPLE: Serum)

Does sleep deprivation lower corticosterone levels in mice?

May 29 2015 | (SPECIES: Mouse, SAMPLE: Serum)

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Arbor Assays will be closed November 25th and 26th for the United States' Thanksgiving holiday.