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Specifications
- Assay Type Competitive ELISA
- Sample Types Plasma, Serum, Saliva, Urine, Fecal Extracts
- Sensitivity 10.6 pg/mL
- Species Cortisone is identical across species
- Assay Duration 2 Hours
- Samples/Plate 40 in Duplicate
- Readout Chemiluminescent
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Standard Curve
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Description
Assay Principle:
The Cortisone Chemiluminescent ELISA Kit quantitatively measures Cortisone in serum, plasma, urine, saliva, and fecal extracts. The Cortisone Chemiluminescent ELISA Kit is a competitive ELISA with a run time of 2 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided cortisone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the cortisone peroxidase conjugate and the cortisone polyclonal rabbit antibody. Then incubate the mixture covered for 2 hours, shaking at room temperature. The immunological reaction occurs between the anti-cortisone polyclonal antibody, the cortisone antigen in the sample or standard, and the cortisone-peroxidase conjugate. As the cortisone concentration in the sample increases, the bound cortisone-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 2-hour incubation, wash away the excess cortisone-peroxidase conjugate and add the chemiluminescent substrate. The chemiluminescent substrate reacts with the bound cortisone-peroxidase conjugate generating a signal detected by a plate reader. Use the intensity and the standard curve to calculate the cortisone concentration in the samples.
Background:
Cortisone (C21H28O5, Kendall’s Compound ‘E’) was by bovine suprarenal gland tissue extraction. Cortisol and cortisone concentrations vary due to the activity of two 11β-hydroxysteroid dehydrogenases (11β-HSD). 11β-HSD1 is found primarily in the liver, which converts Cortisone to cortisol, while 11β-HSD2 is found in tissues such as the kidney where cortisol receptor binding is required. This glucocorticoid “shuttle” helps to initiate and regulate the anti-inflammatory response.
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Structure
