Cortisone

Cortisone was identified by Mason, Myers and Kendall in 1936 as Compound E extracted from bovine suprarenal gland tissue that had the qualitative but not quantitative activity of cortin. The presence of multiple cortin-like compounds led the authors to speculate that the study of Compound E would reveal the nature of cortin. Compound E is now called cortisone and the more active Compound F, cortisol, and the concentrations of these two glucocorticoids vary due to the activity of two 11ß-hydroxysteroid dehydrogenases (11-HSD). While most tissues have the ability to express either enzyme, 11ß-HSD1 is found primarily in the liver where it converts cortisone to cortisol while 11ß-HSD2 is found in tissues such as the kidney where cortisol receptor binding is required. 11ß-HSD2 deactivates cortisol to cortisone, prohibiting receptor activation. This glucocorticoid “shuttle” helps to initiate and regulate the anti-inflammatory response, allowing cortisone to be used as a treatment for a wide range of diseases and conditions.  Monitoring the ratio of cortisone:cortisol has applications in diabetes, obesity, metabolic syndrome, osteoporosis, and chronic fatigue syndrome in addition to adrenal diseases. Cortisone and cortisol concentrations exhibit a predictable diurnal pattern and can be measured in extracted dried feces, or in serum, plasma, saliva and urine. Studies have suggested that salivary cortisone may be a good surrogate marker for serum cortisol.  Arbor Assays offers both a colorimetric EIA kit and a chemiluminescent CLIA Kit to assess cortisone in a variety of samples.

Cortisone EIA Kit, K017-H

Features:

  • USE – Measure Cortisone in 2.5 hours
  • SAMPLE RANGE – 100,000 to under 100 pg/mL
  • SAMPLE – Saliva, Urine, Plasma, Tissue Culture and Dried Fecal Matter
  • SENSITIVITY – 29.0 pg/mL
  • LIMIT OF DETECTION – 60.9 pg/mL
  • STABLITY – Stable 4˚C Liquid Reagents
  • SAMPLES/KIT – 40 or 232 in Duplicate

Cortisone Chemiluminescent Immunoassay Kit, K017-C

Features:

  • USE – Measure Cortisone in 2 hours
  • SAMPLE – Plasma, Serum, Saliva, Urine, Media, and Fecal Extracts
  • SENSITIVITY – 10.6 pg/mL
  • LIMIT OF DETECTION: 16.2 pg/mL
  • SAMPLES/KIT – 37 or 229 in Duplicate
  • STABILITY – Stable 4˚C Liquid Reagents

Related Resources:

Steroid Solid Extraction Protocol

Fecal Extraction Video

Sample Prep Video

Setting up an Arbor Assays EIA Video

CLIA Demonstration Video

Data Analysis Video

Assay Tips and Tricks