Cortisone was identified by Mason, Myers and Kendall in 1936 as Compound E extracted from bovine suprarenal gland tissue that had the qualitative but not quantitative activity of cortin. The presence of multiple cortin-like compounds led the authors to speculate that the study of Compound E would reveal the nature of cortin. Compound E is now called cortisone and the more active Compound F, cortisol, and the concentrations of these two glucocorticoids vary due to the activity of two 11ß-hydroxysteroid dehydrogenases (11-HSD). While most tissues have the ability to express either enzyme, 11ß-HSD1 is found primarily in the liver where it converts cortisone to cortisol while 11ß-HSD2 is found in tissues such as the kidney where cortisol receptor binding is required. 11ß-HSD2 deactivates cortisol to cortisone, prohibiting receptor activation. This glucocorticoid “shuttle” helps to initiate and regulate the anti-inflammatory response, allowing cortisone to be used as a treatment for a wide range of diseases and conditions. Monitoring the ratio of cortisone:cortisol has applications in diabetes, obesity, metabolic syndrome, osteoporosis, and chronic fatigue syndrome in addition to adrenal diseases. Cortisone and cortisol concentrations exhibit a predictable diurnal pattern and can be measured in extracted dried feces, or in serum, plasma, saliva and urine. Studies have suggested that salivary cortisone may be a good surrogate marker for serum cortisol. Arbor Assays offers both a colorimetric EIA kit and a chemiluminescent CLIA Kit to assess cortisone in a variety of samples.
Features:
- USE – Measure Cortisone in 2.5 hours
- SAMPLE RANGE – 100,000 to under 100 pg/mL
- SAMPLE – Saliva, Urine, Plasma, Tissue Culture and Dried Fecal Matter
- SENSITIVITY – 29.0 pg/mL
- LIMIT OF DETECTION – 60.9 pg/mL
- STABLITY – Stable 4˚C Liquid Reagents
- SAMPLES/KIT – 40 or 232 in Duplicate
Cortisone Chemiluminescent Immunoassay Kit, K017-C
Features:
- USE – Measure Cortisone in 2 hours
- SAMPLE – Plasma, Serum, Saliva, Urine, Media, and Fecal Extracts
- SENSITIVITY – 10.6 pg/mL
- LIMIT OF DETECTION: 16.2 pg/mL
- SAMPLES/KIT – 37 or 229 in Duplicate
- STABILITY – Stable 4˚C Liquid Reagents
Related Resources:
Steroid Solid Extraction Protocol
Setting up an Arbor Assays EIA Video