Protein Kinase A (PKA) Colorimetric Activity Kit

Research Areas

Product Protocol Product SDS

Sample Types Cell Lysates, Purified Systems
Time to Answer 3 Hours
Samples/Kit 42 in Duplicate
Stability Fully Active PKA Standard Stored at -20ºC
Readout Colorimetric, 450 nm
Standard Curve
Description
The expressed PKA holoenzyme, comprising of two catalytic (C) and two regulatory (R) subunits, is activated when cAMP levels rise following stimulation of G Protein-coupled receptors and adenylyl cyclase. The phosphorylation of specific substrates by the C subunit of activated PKA is regulated by the subcellular localization of the enzyme through the binding to the scaffolding A kinase-anchoring proteins (AKAPs). In its inactive state, the pseudosubstrate sequences on the R subunits stop the activity of the C subunits. Upon binding of cAMP to the R subunits, the active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC. Substrates that are phosphorylated by PKA include Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3α Ser21 and GSK -3β Ser9). PKA is a pivotal kinase involved in cancer, vasodilation, metabolic processes, etc.

The DetectX® Protein Kinase A (PKA) Activity Kit quantitatively measures PKA activity in a variety of samples.

Sample Types Cell Lysates, Purified Systems
Time to Answer 3 Hours
Samples/Kit 42 in Duplicate
Stability Fully Active PKA Standard Stored at -20ºC
Readout Colorimetric, 450 nm
Standard Curve
Description
The expressed PKA holoenzyme, comprising of two catalytic (C) and two regulatory (R) subunits, is activated when cAMP levels rise following stimulation of G Protein-coupled receptors and adenylyl cyclase. The phosphorylation of specific substrates by the C subunit of activated PKA is regulated by the subcellular localization of the enzyme through the binding to the scaffolding A kinase-anchoring proteins (AKAPs). In its inactive state, the pseudosubstrate sequences on the R subunits stop the activity of the C subunits. Upon binding of cAMP to the R subunits, the active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC. Substrates that are phosphorylated by PKA include Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3α Ser21 and GSK -3β Ser9). PKA is a pivotal kinase involved in cancer, vasodilation, metabolic processes, etc.