Testosterone ELISA Kit


  • Multi Species
  • Use - Non-invasive measurement of Testosterone and Dihydrotestosterone
  • Standard Range 10,000-40.96 pg/mL
  • Sample - Fecal Extracts, Urine, and TCM, as well as extracted Serum and Plasma
  • Samples/Kit - 39 or 231 in Duplicate
  • Stability - Stable 4˚C Liquid Reagents

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The DetectX® Testosterone ELISA Kit uses a specifically generated antibody to measure testosterone and its metabolites in urine and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum, plasma, or saliva samples without extraction. The kit will quantitatively measure Testosterone present in reconstituted buffer samples and tissue culture media samples. A testosterone standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A testosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to testosterone to each well. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound testosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength.

Standard Curve

Data Reduction Template by MyAssays

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+ Publications 32 total publications

Seasonal changes in steroid and thyroid hormone content in shed skins of the tegu lizard Salvator merianea

Aug 11 2021 | (SPECIES: Lizard (Tegu Lizard), SAMPLE: Plasma, Sheds)

Surgical sterilization impacts on behavior of coyote pairs

Jun 24 2021 | (SPECIES: Coyote, SAMPLE: Fecal)

The effect of spinal cord injury on sexual function.

Oct 18 2019 (SPECIES: Rat, SAMPLE: Urine)

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