Specifications

  • Assay Type Competitive ELISA
  • Sample Types Extracted Serum, Extracted Plasma, Fecal Extracts, Urine, Tissue Culture Media
  • Sensitivity 9.92 pg/mL
  • Species Testosterone is identical across all species
  • Assay Duration 2.5 Hours
  • Samples/Plate 39 in Duplicate
  • Readout Colorimetric, 450 nm
  • Standard Curve Testosterone ELISA Kit
  • Description

    Assay Principle: The DetectX® Testosterone ELISA Kit uses a specifically generated antibody to measure testosterone and its metabolites in urine and fecal samples, or in extracted serum and plasma. The kit will quantitatively measure Testosterone present in reconstituted buffer samples and tissue culture media samples. Please read the complete kit insert before performing this assay.

    Background: Testosterone (4-Androsten-17β-ol-3-one) is an anabolic steroid hormone from the androgen group. It is found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, although small amounts are also secreted by the adrenal glands.

    Testosterone is the principal male sex hormone and plays key roles in the development of reproductive tissues such as the testes and prostate. It also promotes secondary sexual characteristics such as increased muscle, bone mass, and body hair. Testosterone plays a significant role in glucose homeostasis, lipid metabolism and the prevention of osteoporosis. Cross-sectional epidemiological studies have reported a direct correlation between plasma testosterone and insulin sensitivity. Low testosterone levels are associated with an increased risk of type 2 diabetes, dramatically illustrated by androgen deprivation in men with prostate carcinoma.

  • Structure Testosterone Chemical Compound