• Assay Type Competitive ELISA
  • Sample Types Extracted Serum, Extracted Plasma, Fecal Extracts, Urine, Tissue Culture Media
  • Sensitivity 9.92 pg/mL
  • Species Testosterone is identical across all species
  • Assay Duration 2.5 Hours
  • Samples/Plate 39 in Duplicate
  • Readout Colorimetric, 450 nm
  • Standard Curve
  • Description

    Assay Principle: 

    The Testosterone ELISA Kit quantitatively measures Testosterone in extracted serum, extracted plasma, fecal extracts, urine, and tissue culture media. The Testosterone ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

    Use our provided Testosterone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the Testosterone peroxidase conjugate and the Testosterone polyclonal rabbit antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-Testosterone polyclonal antibody, the Testosterone antigen in the sample or standard, and the Testosterone-peroxidase conjugate. As the Testosterone concentration in the sample increases, the bound Testosterone-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

    After the 2-hour incubation, wash away the excess Testosterone-peroxidase and add the TMB substrate. The TMB substrate reacts with the bound Testosterone-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the Testosterone concentration in the samples.


    Testosterone (4-Androsten-17β-ol-3-one) is an anabolic steroid hormone from the androgen group, present in mammals, reptiles, birds, and other vertebrates. In mammals, Testosterone is primarily secreted in the testes of males and the ovaries of females, although the adrenal glands also secrete small amounts.

    Testosterone is the principal male sex hormone vital in developing reproductive tissues such as the testes and prostate. It also promotes secondary sexual characteristics such as increased muscle, bone mass, and body hair. Testosterone plays a significant role in glucose homeostasis, lipid metabolism, and the prevention of osteoporosis. Cross-sectional epidemiological studies have reported a direct correlation between plasma testosterone and insulin sensitivity. Low testosterone levels are associated with an increased risk of type 2 diabetes, dramatically illustrated by androgen deprivation in men with prostate carcinoma.


  • Structure Testosterone Chemical Compound