Catalase Fluorescent Activity Kit

Research Areas

Product Protocol Product SDS

Assay Type Activity Assay
Sample Types Serum, Plasma, Cells, Tissues, RBC/Erythrocyte Lysates
Sensitivity 0.073 U/mL
Species Human
Assay Duration 45 Minutes
Samples/Plate 41 in Duplicate
Readout Fluorescent, 590 nm emission / 520 nm excitation
Standard Curve
Description
Hydrogen peroxide, (H2O2) is one of the most frequently occurring reactive oxygen species. It is formed either in the environment, as a by-product of aerobic metabolism, superoxide formation and dismutation, or as a product of oxidase activity. Both excessive hydrogen peroxide and its decomposition product hydroxyl radical, are harmful for most cell components. Its rapid removal is essential for all aerobically living prokaryotic and eukaryotic cells. One of the most efficient ways of removing peroxide is through the enzyme catalase, which is encoded by a single gene and is highly conserved among species. Mammals, including humans and mice, express catalase in all tissues. A high concentration of catalase can be found in the liver, kidneys and erythrocytes. The expression is regulated at transcription, post-transcription and post-translation levels. High catalase activity is detected in peroxisomes.

The DetectX^® Catalase Activity Kit quantitatively measures catalase activity in a variety of samples.

Assay Type Activity Assay
Sample Types Serum, Plasma, Cells, Tissues, RBC/Erythrocyte Lysates
Sensitivity 0.073 U/mL
Species Human
Assay Duration 45 Minutes
Samples/Plate 41 in Duplicate
Readout Fluorescent, 590 nm emission / 520 nm excitation
Standard Curve
Description
Hydrogen peroxide, (H2O2) is one of the most frequently occurring reactive oxygen species. It is formed either in the environment, as a by-product of aerobic metabolism, superoxide formation and dismutation, or as a product of oxidase activity. Both excessive hydrogen peroxide and its decomposition product hydroxyl radical, are harmful for most cell components. Its rapid removal is essential for all aerobically living prokaryotic and eukaryotic cells. One of the most efficient ways of removing peroxide is through the enzyme catalase, which is encoded by a single gene and is highly conserved among species. Mammals, including humans and mice, express catalase in all tissues. A high concentration of catalase can be found in the liver, kidneys and erythrocytes. The expression is regulated at transcription, post-transcription and post-translation levels. High catalase activity is detected in peroxisomes.