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- Assay Type Competitive ELISA
- Sample Types Plasma, Urine, Tissue Culture Media
- Sensitivity 0.49 ng/mL
- Species Human, Pig, Sheep, Dog, Rat, Mouse
- Assay Duration 1.5 Hours
- Samples/Plate 40 in Duplicate
- Readout Colorimetric, 450 nm
- Standard Curve
The Atrial Natriuretic Peptide (ANP) ELISA Kit quantitatively measures ANP in plasma, urine, and tissue culture media. The Atrial Natriuretic Peptide (ANP) ELISA Kit is a competitive ELISA with a run time of 1.5 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided ANP standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the ANP peroxidase conjugate and the ANP rabbit polyclonal antibody. Then incubate the mixture covered at room temperature, shaking for 1 hour. The immunological reaction occurs between the anti-ANP antibody, the ANP antigen in the sample or standard, and the ANP conjugate. As the ANP concentration in the sample increases, the bound ANP-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 1-hour incubation, wash away the excess ANP-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound ANP-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the ANP concentration in the samples.
Atrial natriuretic peptide (ANP), a peptide hormone secreted mainly through cardiac myocytes, is a potent natriuretic, diuretic, and vasodilatory peptide contributing to blood pressure and volume homeostasis. Myocytes release ANP in response to atrial distension. Upon binding to cell surface receptors (NPR-A, B, and C, also termed guanylyl cyclase-A and B receptors), ANP acts through the generation of cyclic guanosine monophosphate (cGMP). Atrial natriuretic peptide demonstrates hemodynamic and glomerular effects, which increase sodium and water load delivery to the tubules and inhibit the release of renin, aldosterone, and vasopressin.