Assay Principle: 

The Atrial Natriuretic Peptide (ANP) ELISA Kit quantitatively measures ANP in plasma, urine, and tissue culture media. This competitive ELISA has a run time of 1.5 hours. Please read the complete kit insert for more information before performing this assay. Use our provided ANP standard to generate a standard curve for the assay.

Protocol Summary:

• Introduce standards or diluted samples into the transparent microtiter plate pre-coated with goat anti-rabbit IgG antibody.
• Add the ANP peroxidase conjugate and the ANP rabbit polyclonal antibody to initiate the immunological reaction.
• Incubate the mixture covered at room temperature with shaking for 1 hour. The reaction is inversely proportional to the ANP concentration in the sample.
• Post incubation, wash away excess conjugate and add the TMB substrate, which reacts with the bound conjugate to produce a measurable signal.
• Use a plate reader to detect the signal at 450nm and calculate the ANP concentration based on the standard curve.

Background:

Atrial Natriuretic Peptide (ANP) is a cardiac hormone predominantly secreted by myocytes in the heart’s atria. It plays a vital role in regulating cardiovascular homeostasis by exerting potent natriuretic, diuretic, and vasodilatory effects, crucial for maintaining blood pressure and fluid balance.

ANP is a preprohormone, synthesized and released in response to atrial distension resulting from increased blood volume. Once secreted, ANP binds to specific cell surface receptors, primarily NPR-A (natriuretic peptide receptor A) and NPR-B. This binding triggers intracellular cyclic guanosine monophosphate (cGMP) production, which mediates the hormone’s various physiological actions.

The diuretic and natriuretic effects of ANP are characterized by increased renal sodium and water excretion, contributing to decreased blood volume and blood pressure. Additionally, ANP inhibits the renin-angiotensin-aldosterone system (RAAS) and counteracts the effects of vasopressin, further aiding in blood pressure regulation. It also possesses vasodilatory properties, leading to the relaxation of vascular smooth muscle cells.

Clinically, ANP levels are of significant interest in diagnosing and managing various cardiovascular conditions, including heart failure, hypertension, and atrial fibrillation. Patients with heart failure often have elevated levels of ANP, which acts as a compensatory mechanism against volume overload.

The DetectX® Atrial Natriuretic Peptide (ANP) ELISA Kit provides a precise and efficient method for quantifying ANP levels in plasma, urine, and tissue culture media. This kit is an essential tool for cardiovascular researchers, offering insights into the pathophysiology of heart diseases and aiding in the assessment and management of cardiovascular disorders.