Specifications

  • Use Kidney Damage Assessment
  • Sample Types Serum and Plasma (EDTA and Heparin)
  • Species Human, Mouse, Rat, Rabbit, Sheep
  • Samples/Kit 187 in Duplicate
  • Calibrated N-Cal Kit, NIST-Calibrated
  • Stability Stable 4°C Liquid Reagents
  • Readout Colorimetric, 490 nm
  • Standard Curve
  • Description

    Creatinine is a metabolite of phosphocreatine (p-creatine), a high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH. Measuring serum creatinine is one of the most commonly used indicators of renal function. A rise in blood creatinine levels is observed only with marked damage to functioning nephrons. A good indicator of kidney function is given by the creatinine clearance test. Creatinine clearance can be accurately calculated using serum creatinine concentration and taking into account the variables of sex, age, weight, and race.