Levonorgestrel (LNG) ELISA Kit

Assay Principle: 

The DetectX® Levonorgestrel (LNG) ELISA Kit quantitatively measures LNG in saliva, urine, water, milk, tissue culture media, extracted serum, plasma (EDTA and Heparin), and fecal extracts. This competitive ELISA has a run time of 1.5 hours. Please read the complete kit insert for more information before performing this assay. Use our provided LNG standard to generate a standard curve for the assay.

Protocol Summary:

• Introduce standards or diluted samples into the provided transparent microtiter plate, pre-coated with goat anti-rabbit IgG antibody.
• Add LNG peroxidase conjugate and LNG polyclonal rabbit antibody to the wells, ensuring a thorough mix.
• Incubate the mixture at room temperature, covered and shaking, for 1 hour. The immunological reaction varies inversely with the LNG concentration in the sample.
• After incubation, remove the excess conjugate and introduce the TMB substrate. The substrate reacts with the bound conjugate, producing a detectable colorimetric signal.
• Measure the signal intensity at 450nm using a plate reader and calculate the LNG concentration based on the standard curve.

Background:

Levonorgestrel (LNG) is a synthetic progestin widely used in contraceptives, treating dysmenorrhea, and providing endometrial protection in estrogen replacement therapy for postmenopausal women. Its diverse applications in gynecology include management of heavy menstrual bleeding, pelvic pain, endometrial hyperplasia, and early-stage endometrial cancer.

LNG’s contraceptive action works by inhibiting ovulation, altering cervical mucus to prevent sperm passage, and modifying the uterine lining. It is also deemed safe for use during breastfeeding. Quantifying LNG levels in biological samples is important for assessing therapeutic efficacy and ensuring optimal drug concentrations.

Additionally, environmental monitoring of LNG, using this ELISA kit, helps mitigate potential toxic effects on aquatic ecosystems. This aspect is particularly important in assessing the environmental impact of contraceptive manufacturing, use, and disposal.

The DetectX® Levonorgestrel (LNG) ELISA Kit offers a sensitive and specific method for measuring LNG levels in diverse sample types for clinical research, environmental monitoring, and pharmacological studies.