- Most Sensitive
- Multi Species
- Use - Measure LNG in a variety of matrices
- Sample - Saliva, Urine, Water, Milk, TCM, and Extracted Serum, Plasma or Fecal Material
- Samples/Kit - 40 or 232 in Duplicate
- Stability - Liquid 4˚C Stable Reagents
The DetectX® Levonorgestrel (LNG) ELISA Kit is designed to quantitatively measure Levonorgestrel present in serum, plasma, saliva, urine, dried fecal samples, water, and tissue culture media samples. This kit measures total LNG in extracted serum, plasma and fecal samples.
A levonorgestrel stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A levonorgestrel-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody to levonorgestrel to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound levonorgestrel-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the levonorgestrel in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.