Myeloperoxidase (MPO) Human ELISA Kit

Research Areas

Documentation

Specifications

• Assay Type Sandwich ELISA
• Sample Types Serum, Platelet-Poor Heparin Plasma, Saliva, Urine, Tissue Culture Media
• Sensitivity 68 pg/mL
• Species Human
• Assay Duration 2.5 Hours
• Samples/Plate 40 in duplicate
• Readout Colorimetric, 450 nm
• Standard Curve
• Description

  Assay Principle: 

  The Myeloperoxidase (MPO) Human ELISA Kit quantitatively measures MPO levels in serum, platelet-poor heparin plasma, saliva, urine, and tissue culture media. The Myeloperoxidase (MPO) Human ELISA Kit is a sandwich ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

  Use our provided MPO standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our anti-human MPO monoclonal antibody. Incubate the covered plate shaking for 1 hour at room temperature. Wash the plate and add the peroxidase-conjugated human MPO antibody. Then incubate the mixture covered at room temperature, shaking for 1 hour. The immunological reaction occurs between the peroxidase-conjugated human MPO antibody and the MPO antigen in the sample or standard. 

  After the 1-hour incubation, wash away the excess peroxidase-conjugated human MPO antibody and add the TMB substrate. The TMB substrate reacts with the bound peroxidase-conjugated MPO antibody generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the MPO concentration in the samples.

  Background:

  Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes, where it plays a critical anti-microbial role. Typically stored in azurophilic granules, MPO is released into the extracellular space during degranulation. As part of the neutrophils’ “respiratory burst,” it produces hypochlorous acid from hydrogen peroxide and Cl-. MPO also uses hydrogen peroxide to oxidize tyrosine to the tyrosyl radical. Both hypochlorous acid and tyrosyl are cytotoxic and, when present, can kill bacteria and other pathogens. Hereditary deficiency of myeloperoxidase predisposes individuals to immune deficiency.

  The clinical utility of MPO testing in cardiac patients is well-established in the literature, with well over 100 papers published. Studies have shown an association between elevated MPO levels and coronary artery disease, suggesting that MPO may be a sensitive predictor of myocardial infarction in patients complaining of chest pain. In 2010 this clinical application was further refined by additional studies, which determined that measuring both MPO and C-reactive protein (CRP) provided a more accurate prediction of mortality risk than measuring just CRP alone.