Prolactin (PRL) ELISA Kit

Research Areas

Documentation

Specifications

• Assay Type Sandwich ELISA
• Sample Types Serum, Plasma, Tissue Culture Media
• Sensitivity 11.7 pg/mL
• Species Human, Elephant, Seal
• Assay Duration 2.5 Hours
• Samples/Plate 40 in Duplicate
• Readout Colorimetric, 450 nm
• Standard Curve
• Description

  Assay Principle: 

  The Prolactin (PRL) ELISA Kit quantitatively measures Prolactin levels in serum, plasma, and tissue culture media. The Prolactin (PRL) ELISA Kit is a sandwich ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

  Use our provided Prolactin standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our mouse anti-Prolactin antibody and incubate shaking at room temperature for 1 hour. Add the peroxidase-conjugated human Prolactin polyclonal antibody. Then incubate the mixture covered, shaking at room temperature for 1 hour. The immunological reaction occurs between the peroxidase-conjugated Prolactin polyclonal antibody and the Prolactin antigen in the sample or standard. 

  After the 1-hour incubation, wash away the excess peroxidase-conjugated Prolactin antibody and add the TMB substrate. The TMB substrate reacts with the bound peroxidase-conjugated Prolactin antibody generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the Prolactin concentration in the samples.

  Background:

  Prolactin (PRL) is a polypeptide hormone synthesized and secreted from specialized cells of the anterior pituitary gland. This hormone was named Prolactin because an extract of the bovine pituitary gland would cause the crop sac’s growth and stimulate milk production in pigeons or promote lactation in rabbits. However, Prolactin has over 300 different biological activities. Prolactin has multiple roles in reproduction besides lactation and plays multiple homeostatic functions in the organism. Furthermore, numerous other organs and tissues besides the anterior pituitary can synthesize and secrete Prolactin. 

  Profile of serum Prolactin levels from a pregnant African Elephant