TBARS/MDA Universal Colorimetric Detection Kit (2 Plates)

Research Areas

Product Protocol Product SDS

Sample Types Serum, Plasma, Tissue, Cell Extracts, Food Extracts, Urine, Buffers
Sensitivity 0.36 µM
Time to Answer 1 Hour
Format Shaking at 37˚C
Samples/Kit 88 in duplicate
Stability Liquid 4ºC stable reagents
Readout Colorimetric, 535 nm
Standard Curve
Description
Lipid peroxidation is a well-established mechanism of cellular injury in plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxidation products derived from polyunsaturated fatty acids are stable and decompose to form a diverse mixture of compounds, including MDA.

MDA is conveniently measured by the reaction of thiobarbituric acid in an acid environment according to the following reaction.

The MDA-TBA adduct formed in this reaction is pink-colored and can be read at ƛ= 532 nm. Measurement between 530–545 nm is acceptable.

The DetectX^® TBARS/MDA Universal Colorimetric Detection Kit allows assessment of lipid peroxidation without boiling water bath treatment.

Sample Types Serum, Plasma, Tissue, Cell Extracts, Food Extracts, Urine, Buffers
Sensitivity 0.36 µM
Time to Answer 1 Hour
Format Shaking at 37˚C
Samples/Kit 88 in duplicate
Stability Liquid 4ºC stable reagents
Readout Colorimetric, 535 nm
Standard Curve
Description
Lipid peroxidation is a well-established mechanism of cellular injury in plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxidation products derived from polyunsaturated fatty acids are stable and decompose to form a diverse mixture of compounds, including MDA.

MDA is conveniently measured by the reaction of thiobarbituric acid in an acid environment according to the following reaction.

The MDA-TBA adduct formed in this reaction is pink-colored and can be read at ƛ= 532 nm. Measurement between 530–545 nm is acceptable.