- Multi Species
- Use - Assess lipid peroxidation without boiling water bath treatment
- Sample - Serum, Plasma, Tissue, Cell and Food Extracts, Urine, and Buffers
- Samples/Kit - 88 in duplicate
- Sensitivity - 0.36 µM
- Stability - Liquid 4˚C Stable Reagents
- Assay Format - 1 hour 37˚C shaking
Lipid peroxidation is a well-established mechanism of cellular injury in plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxidation products derived from polyunsaturated fatty acids are stable and decompose to form a diverse mixture of compounds, including MDA.
MDA is conveniently measured by the reaction of thiobarbituric acid in an acid environment according to the following reaction.
The MDA-TBA adduct formed in this reaction is pink-colored and can be read at ƛ= 532 nm. Measurement between 530–545 nm is acceptable.