Specifications

  • Sample Serum, Plasma, Tissue, Cell and Food Extracts, Urine, Buffers
  • Sensitivity 0.36 µM
  • Time to Answer 1 Hour
  • Format Shaking at 37˚C
  • Samples/Kit 88 in duplicate
  • Stability Liquid 4ºC stable reagents
  • Readout Colorimetric, 535 nm
  • Standard Curve K077-H Standard Curve
  • Description

    Lipid peroxidation is a well-established mechanism of cellular injury in plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxidation products derived from polyunsaturated fatty acids are stable and decompose to form a diverse mixture of compounds, including MDA.

    MDA is conveniently measured by the reaction of thiobarbituric acid in an acid environment according to the following reaction.

    The MDA-TBA adduct formed in this reaction is pink-colored and can be read at ƛ= 532 nm. Measurement between 530–545 nm is acceptable.