Arbor Assays’ New TXB2 ELISA Kit is Here: Upgrade Your Platelet and Cardiovascular Research!
Biomarker research is essential in advancing our understanding of various physiological and pathological processes. With the launch of the DetectX®...
Specifications
- Catalog Numbers K092-H1, K092-H5
- Assay Type Competitive ELISA
- Sample Types Serum, Plasma, Urine, Tissue Culture Media
- Sensitivity 11.2 pg/mL
- Species Thromboxane B2 is identical across species
- Assay Duration 2.5 Hours
- Samples/Plate 39 in Duplicate
- Readout Colorimetric, 450 nm
Images
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TXB2 Standard Curve
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Assay Principle
The DetectX® Thromboxane B2 (TXB2) ELISA Kit quantitatively measures TXB2 in serum, plasma, urine, and tissue culture media samples. The TXB2 ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.
A TXB2 Standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a polyclonal antibody to capture rabbit antibodies. A TXB2-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to TXB2 to each well. As the TXB2 concentration in the sample increases, the bound TXB2-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After an incubation, the plate is washed and substrate is added. The substrate reacts with the bound TXB2-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the TXB2 in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers.
Background
Thromboxane B2 (TXB2), C20H34O6, is a stable metabolite produced by Thromboxane A2 (TXA2) involved in platelet activation and aggregation. The eicosanoid TXA2 is the predominant product of cyclooxygenase, specifically COX-1. COX-1 catalyzes the first two steps of prostaglandins (PGs) biosynthesis. PGs play the key role in generation of an inflammatory response and are primary targets for nonsteroidal anti-inflammatory drugs (NSAIDs). TXA2 itself has prothrombotic properties and is a known vasoconstrictor. It is also thought to play a role in the pathogenesis of myocardial infarction, stroke, atherosclerosis, and bronchial asthma. TXA2 is extremely unstable, with a half-life of 30 seconds. Therefore TXB2, after it is hydrated from active TXA2, is the ideal candidate for a stable metabolite biomarker to use for an abundance of conditions and measuring anti-platelet drug effectiveness.