Nitric oxide (NO) is a diffusible, transient, reactive molecule, and an important regulator of cardiovascular, neurological, and immunological systems. NO is generated endogenously by constitutive or induced enzymes like Nitric Oxide Synthase or orally ingested as nitrates/nitrites for rapid uptake into circulation and subsequent conversion.
The physical properties of NO make it challenging for direct detection methods. Thankfully, colorimetric methods can be used to measure the stable break-down products, nitrate and nitrite.
- Use – Measure Nitrite in 5 Minutes, Total Nitric Oxide in 30 Minutes
[Nitrate] = [Total Nitric Oxide] – [Nitrite]
- N-Cal – NIST-Calibrated and ISO11EC-calibrated standard solutions
- Sample – Water, Serum, Plasma, Urine, Saliva, Buffers, and TCM
- Sensitivity – 2.63 μM (Nitrite) and 1.02 μM (Total Nitric Oxide)
- Samples/Kit – Up to 88 in Duplicate with 2 plates
- Species – Independent
- Click Here To View Citations
Typical Standard Curve