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Specifications

  • Assay Type Detection Kit
  • Sample Types Water, Serum, Plasma, Urine, Saliva, Lysates, Buffers, Tissue Culture Media
  • Sensitivity 2.63 µM (Nitrite), 1.02 µM (Total NO)
  • Species NO, Nitrate, and Nitrite are identical across species
  • Assay Duration 5 (Nitrite), 25 Minute (Total NO)
  • Samples/Plate 40 in Duplicate
  • Readout Colorimetric, 550-570 nm
  • Standard Curve
  • Description

    Nitric oxide (NO) is a diffusible, transient, reactive molecule that has physiological effects in the pM-µM range. Acting through guanylate cyclase activation, NO is an important regulator of the cardiovascular, nervous, and immunological systems. NO is bio-available by two routes. It can be endogenously generated by constitutive or induced NOS enzymes, or it can be ingested as nitrates or nitrites for conversion into NO.

    The reactive nature of nitric oxide allows it to act as a cytotoxic factor when released during an immune response by macrophages. The reactivity also allows NO to be easily converted to a toxic radical that can produce nitrosylation damage to cells and DNA. Nitrosylation can be a regulated post-translational modification in cell signaling. The dynamics of the regulatory/damage facets of NO are major forces in mitochondrial signaling and dysfunction. NO is linked not only to coronary heart disease, endothelial dysfunctions, erectile dysfunction, and neurological disorders, but also diabetes, chronic periodontitis, autism and cancer.

    This is a multi-format kit that detects Nitrite and total Nitric Oxide in a variety of sample types.