Cortisol EIA Kit

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  • USE - Measure Cortisol in 90 Minutes
  • SAMPLE - Fecal Extracts, Urine, Serum, Plasma, Saliva and TCM
  • SAMPLES/KIT -39 or 231 in Duplicate
  • CALIBRATED - N-Cal Kit, NIST-Calibrated
  • STABILITY - Stable 4˚C Liquid Reagents

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Multi Species and N-Cal Kit


The DetectX® Cortisol Enzyme Immunoassay kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and culture media samples. This kit measures total cortisol in extracted samples, serum and plasma and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol to each well. After an 1 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation the color reaction is read at 450nm.

Cortisol is the primary glucocorticoid produced and secreted by the adrenal cortex. It is often referred to as the “stress hormone” as it is involved in the response to stress and it affects blood pressure, blood sugar levels, and other actions of stress adaptation. Immunologically, cortisol functions as an important anti-inflammatory and plays a role in hypersensitivity, immunosuppression, and disease resistance. In the metabolic aspect, cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Production of cortisol follows an ACTH-dependent circadian rhythm, with a peak level in the morning and decreasing levels throughout the day. Most serum cortisol, all but about 4%, is bound to proteins including corticosteroid binding globulin and serum albumin. Only free cortisol is available to most receptors and it is through these receptors that physiological processes are modulated. Abnormal cortisol levels are being evaluated for correlation with a variety of different conditions, such as prostate cancer, depression, and schizophrenia. It is already known that abnormal levels of cortisol are involved in Cushing’s Syndrome and Addison’s disease.

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Item Catalog # Price
Cortisol EIA Kit, 1 Strip-Well PlateK003-H1$220.00Inquire About This Product
Cortisol EIA Kit, 5 Strip-Well PlateK003-H5$875.00Inquire About This Product
Cortisol EIA Kit, 1 Solid PlateK003-H1W$220.00Inquire About This Product
Cortisol EIA Kit, 5 Solid PlateK003-H5W$875.00Inquire About This Product


Date Publication
Jul 08 2017 Initial validation of blubber cortisol and progesterone as indicators of stress response and maturity in an Otariid; The California Sea Lion (Zalophus californianus)
Jul 08 2017 Stress differentially affects the systemic and leukocyte estrogen network in common carp
May 26 2017 Comparison of productive and reproductive performance and hair cortisol levels between Brown Swiss cross-bred and Holstein cows housed in the same barn
May 13 2017 Wagging more, barking less: Glucocorticoid and behavioral responses of shelter dogs to human interaction
May 01 2017 Growth Patterns and Size-Group Interactions in Hatchery-Reared Age-0 Lake Sturgeon
Mar 16 2017 Stress assessment using hair cortisol of kangaroos affected by the lumpy jaw disease
Feb 14 2017 Aversiveness of husbandry procedures for pre-weaned foals: A comparison using behavioural and physiological indices
Feb 01 2017 Use of fecal glucocorticoid and salivary cortisol concentrations as a measure of well-being of New York City carriage horses
Jan 20 2017 Influence of exposure to coarse, fine and ultrafine urban particulate matter and their biological constituents on neural biomarkers in a randomized controlled crossover study
Jan 11 2017 Space allowance influences individually housed Holstein bull calf innate immune measures and standing behaviors after castration at 3 weeks of age
Dec 23 2016 A Reusable Electrochemical Biosensor for Monitoring of Small Molecules (Cortisol) Using Molecularly Imprinted Polymers
Nov 29 2016 Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil
Nov 17 2016 A novel method for assessing chronic cortisol concentrations in dogs using the nail as a source
Sep 27 2016 Effects of dietary yeast inclusion and acute stress on post- prandial whole blood profiles of dorsal aorta-cannulated rainbow trout
Sep 13 2016 Blubber cortisol qualitatively reflects circulating cortisol concentrations in bottlenose dolphins
Sep 07 2016 Evaluation of serum protein-based arrival formula and serum protein supplement (Gammulin) on growth, morbidity, and mortality of stressed (transport and cold) male dairy calves
Aug 17 2016 Hair plucking, stress, and urinary cortisol among captive bonobos (Pan paniscus)
Aug 03 2016 Get the most out of blow hormones: validation of sampling materials, field storage and extraction techniques for whale respiratory vapour samples
Aug 01 2016 Evaluation of hematological and serum biochemical profiles associated with the supplementation of zilpaterol hydrochloride to feedlot heifers
Aug 01 2016 Evaluation of the metabolic response, blood chemistry profile, and cardiac morphology of beef heifers supplemented with zilpaterol hydrochloride followed by an endocrine induced stress challenge
Jul 06 2016 Sonochemical Synthesis of Zinc Oxide Nanostructures for Sensing and Energy Harvesting
Jun 01 2016 Understanding Mental Toughness and Stress: The Role of Cortisol
May 06 2016 Effects of culling on vigilance behaviour and endogenous stress response of female fallow deer
Apr 20 2016 Evaluation of the innate immune response of Angus heifers with genetic marker variation for intramuscular fat deposition following a lipopolysaccharide challenge
Mar 07 2016 Addition of a dairy fraction rich in milk fat globule membrane to a high-saturated fat meal reduces the postprandial insulinaemic and inflammatory response in overweight and obese adults
Mar 04 2016 Cattle temperament influences metabolism: metabolic response to glucose tolerance and insulin sensitivity tests in beef steers
Feb 29 2016 Interaction of Biological Stress Recovery and Cognitive Vulnerability for Depression in Adolescence
Feb 03 2016 ATR-101 disrupts mitochondrial functions in adrenocortical carcinoma cells and in vivo
Jan 14 2016 Compounds and Methods for treating aberrant adrenocortical cell disorders
Jan 14 2016 An in vitro reproduction of stress-induced memory defects: Effects of corticoids on dendritic spine dynamics
Dec 03 2015 Antibodies to microbiome, stress factors and mast cell markers as diagnostic markers for IBS
Oct 09 2015 Welfare of a pair of Captive Tigers: a Hand-Reared Female and a Parent-Reared Male
Oct 05 2015 Celecoxib reduces glucocorticoids in vitro and in a mouse model with adrenocortical hyperplasia
Oct 01 2015 Emotional Reactivity and Internalizing Symptoms: Moderating Role of Emotion Regulation
Sep 04 2015 Glucocorticoid receptor expression and binding capacity in patients with burn injury
Sep 03 2015 Label-free Electrochemical Biosensor
Aug 05 2015 Salivary but not plasma cortisone tracks the plasma cortisol response to exercise: effect of time of day
Aug 04 2015 The Effects of Socializing and Environmental Enrichments on Sow and Piglet Behavior and Performance
Jul 28 2015 Impairment of neutrophilic glucocorticoid receptor function in patients treated with steroids for septic shock
Jun 04 2015 Activity of the hypothalamus–pituitary–interrenal axis (HPI axis) and immune response in carp lines with different susceptibility to disease
Mar 27 2015 Stress and the Multiple-Role Woman: Taking a Closer Look at the “Superwoman”
Mar 25 2015 Physiological Plasticity to Water Flow Habitat in the Damselfish, Acanthochromis polyacanthus: Linking Phenotype to Performance
Mar 02 2015 Hair cortisol detection in dairy cattle by using EIA: protocol validation and correlation with faecal cortisol metabolites
Mar 02 2015 Set-up of a multivariate approach based on serum biomarkers as an alternative strategy for the screening evaluation of the potential abuse of growth promoters in veal calves
Feb 07 2015 New Route of Investigation for Understanding the Impact of Human Activities on the Physiology of Non-Human Primates
Feb 02 2015 Blubber Cortisol: A Potential Tool for Assessing Stress Response in Free-Ranging Dolphins without Effects due to Sampling
Oct 31 2014 Hypothalamic-pituitary-adrenal axis dysfunction and illness progression in bipolar disorder
Aug 08 2014 Haematological and physiological responses of piemontese beef cattle to different housing conditions
Jul 22 2014 Intestinal steroidogenesis controls PPARγ expression in the colon and is impaired during UC
Jul 17 2014 Electrochemical cortisol immunosensors based on Sonochemically synthesized zinc oxide 1D nanorods and 2D nanoflakes
Jun 27 2014 Baleen hormones: a novel tool for retrospective assessment of stress and reproduction in bowhead whales (Balaena mysticetus)
Jun 01 2014 Innate humoral immune parameters in Tilapia zillii under acute stress by low temperature and crowding
Apr 24 2014 Follow-up in healthy schoolchildren and in adolescents with DOWN syndrome: psycho-environmental and genetic determinants of physical activity and its impact on fitness, cardiovascular diseases, inflammatory biomarkers and mental health; the UP&DOWN Study
Mar 31 2014 Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m
Mar 09 2014 Effects of ractopamine or zilpaterol on phy siologic and metabolic parameters in feedlot steers
Feb 24 2014 Metabolic crosstalk between the heart and liver impacts familial hypertrophic cardiomyopathy
Feb 01 2014 Behavior and performance of veal calves in relation to group housing
Dec 24 2013 Electrochemical Immunosensing of Saliva Cortisol
Oct 10 2013 Compounds and Methods for Treating Aberrant Adrenocartical Cell Disorders
Oct 02 2013 Detection of steroid and thyroid hormones via immunoassay of North Atlantic right whale (Eubalaena glacialis) respiratory vapor
Jan 03 2013 Yeast cell wall supplementation alters aspects of the physiological and acute phase responses of crossbred heifers to an endotoxin challenge
Aug 30 2012 Yeast Cell Wall Supplementation Alters the Performance and Health of Newly Received Crossbred Heifers
Mar 15 2012 Spinal-General Anaesthesia Decreases Neuroendocrine Stress Response in Laparoscopic Cholecystectomy.
Dec 16 2011 Enhancement of the acute phase response to a lipopolysaccharide challenge in steers supplemented with chromium.

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