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- Assay Type Competitive ELISA
- Sample Types Serum, Plasma, Saliva, Urine, Tissue Culture, Respiratory Vapor, Hair, Fecal Extracts
- Sensitivity 27.6 pg/mL
- Species Cortisol is identical across species
- Assay Duration 1.5 Hours
- Samples/Plate 39 in Duplicate
- Readout Colorimetric, 450 nm
- Standard Curve
The Cortisol ELISA Kit quantitatively measures cortisol in saliva, urine, serum, plasma, dried fecal extracts, and tissue culture media samples. The Cortisol ELISA Kit is a competitive ELISA with a run time of 1.5 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided cortisol standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-mouse IgG antibody. Add the cortisol peroxidase conjugate and the cortisol monoclonal mouse antibody. The immunological reaction occurs between the anti-cortisol monoclonal antibody, the cortisol antigen in the sample or standard, and the cortisol-peroxidase conjugate. Then incubate the mixture for 1 hour shaking at room temperature. As the cortisol concentration in the sample increases, the bound cortisol-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 1 hour incubation, wash away the excess cortisol-peroxidase and add the TMB substrate. The TMB substrate reacts with the bound cortisol-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the cortisol concentration in the samples.
Performance: The Cortisol ELISA Kit is one of the most sensitive cortisol assays on the market, delivering results in as little as 90 minutes using a standard plate reader. The cortisol assay is compatible with a wide variety of sample types from various species and utilizes as little as 1μL of Serum or Plasma to perform the assay.
Cortisol is the primary glucocorticoid produced and secreted by the adrenal cortex. Production of cortisol follows an ACTH-dependent circadian rhythm, with a peak level in the morning and decreasing levels throughout the day. Most of this circulating cortisol is bound to proteins such as corticosteroid-binding globulin and serum albumin. Only free cortisol is available to interact with most receptors and modulate physiological processes.
Often referred to as the “stress hormone,” it affects blood pressure, blood sugar levels, and other actions of stress adaptation. Cortisol is an anti-inflammatory hormone playing roles in hypersensitivity, immunosuppression, and disease resistance. Cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Abnormal cortisol levels correlate with Prostate Cancer, Depression, Schizophrenia, Cushing’s Syndrome, and Addison’s Disease.