Nitric Oxide (NO) Colorimetric Detection Kit

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  • USE - Measure Nitrite in 5 Minutes, Total NO in 30 Minutes
  • SAMPLE - Water, Serum, Plasma, Urine, Saliva, Buffers and TCM
  • SAMPLES/KIT - 88 in Duplicate
  • CALIBRATED - N-Cal kit, NIST-Calibrated
  • STABILITY - Non-Toxic, Stable 4˚C Liquid Reagents

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Multi Species, N-Cal Kit, Most Sensitive


The DetectX® Nitric Oxide (NO) Detection Kit is designed to quantitatively measure Nitrate and Nitrite present in a variety of samples. Nitric Oxide content is derived from the sum of Nitrate and Nitrite. Both Nitrate and Nitrite standards are provided to generate standard curves for the assay and all samples should be read off the appropriate standard curve. For Nitrite Detection, samples are mixed with the Color Reagents A and B and incubated at room temperature for 5 minutes. The colored product is read at 550–570 nm.

Total Nitric Oxide content is measured after the sample is incubated with Nitrate Reductase and NADH. The reductase in combination with NADH reduces Nitrate to Nitrite. After a 20 minute incubation at room temperature, Color Reagents A and B are added and incubated at room temperature for 5 minutes. The concentration of Nitrate in the sample is calculated by taking the measured Nitrite concentration and subtracting from the Total Nitric Oxide concentration for the sample.

Nitric Oxide Standard Curves

Data Reduction Template by MyAssays

Item Catalog # Price
Nitric Oxide Colorimetric Detection Kit, 2 PlateK023-H1$210.00Inquire About This Product


Date Publication
Jul 05 2017 Effect of one-year oral supplementation with vitaminized olive oil on platelets from healthy post-menopausal women
Apr 05 2017 Investigation of the role of nitric oxide driven angiogenesis by zinc oxide nanoflowers
Feb 16 2017 A pilot randomised controlled trial investigating a Mediterranean diet intervention in pregnant women for the primary prevention of allergic diseases in infants
Dec 30 2016 Different transcriptional profiling between senescent and non-senescent human coronary artery endothelial cells (HCAECs) by Omeprazole and Lansoprazole treatment
Oct 10 2016 Impaired platelet activation in patients with hereditary deficiency of p47 phox
May 19 2016 Short-term ubiquinol supplementation reduces oxidative stress associated with strenuous exercise in healthy adults: A randomized trial
Apr 19 2016 In vitro lifespan and senescent behaviour of human periosteal derived stem cells
May 15 2015 Effect of estradiol and its metabolites on nitrosative stress induced by chromiumVI
May 15 2015 Characterization of diabetic osteoarthritic cartilage and role of high glucose environment on chondrocyte activation: toward pathophysiological delineation of diabetes mellitus-related osteoarthritis
May 11 2015 Altered activities of kininase II, an angiotensin converting enzyme, prekallikrein, and nitric oxide in Kuwaiti patients with type 2 diabetes
Apr 27 2015 Abnormalities associated with progressive aortic vascular dysfunction in chronic kidney disease
Apr 20 2015 Hyperglycaemia Enhances Nitric Oxide Production in Diabetes: A Study from South Indian Patients
Sep 20 2014 Human Periosteal Derived Stem Cell Potential: The Impact of aging
Nov 01 2013 The Effects of Coenzyme Q10 and Baicalin in Cisplatin-Induced Lipid peroxidation and Nitrosative Stress
Jul 17 2012 Regulatory T lymphocytes from ALS mice suppress microglia and effector T lymphocytes through different cytokine-mediated mechanisms.

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