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- Sample Serum, Plasma
- Sensitivity 0.053 µg/mL
- Range 20.0-0.313 µg/mL
- Time to Answer 30 minutes
- Stability Liquid 4ºC stable reagents
- Samples/Kit 88 or 472 in Duplicate
- Readout 450 nm
- Standard Curve
All forms of hemoglobin react with Hemoglobin Detection Reagent and the generated color is measured photometrically at 450 nm. A human hemoglobin standard is provided to generate a standard curve for the assay.
Hemoglobin (Hgb) is an erythrocyte protein complex comprised of two sets of identical pairs of subunits, each of which bind an iron-porphyrin group commonly called heme. Heme binds and releases oxygen or carbon dioxide in response to slight changes in local gas tension. Hemoglobin values are associated with a variety of conditions ranging from anemias (low Hgb), erythrocytosis (high Hgb), thalassemias (aberrant chain synthesis), and sickling disorders (abnormal complex shape).