Specifications

  • Sample Serum, Plasma
  • Sensitivity 0.053 µg/mL
  • Range 20.0-0.313 µg/mL
  • Time to Answer 30 minutes
  • Stability Liquid 4ºC stable reagents
  • Samples/Kit 88 or 472 in Duplicate
  • Readout 450 nm
  • Standard Curve
  • Description

    All forms of hemoglobin react with Hemoglobin Detection Reagent and the generated color is measured photometrically at 450 nm. A human hemoglobin standard is provided to generate a standard curve for the assay.

    Hemoglobin (Hgb) is an erythrocyte protein complex comprised of two sets of identical pairs of subunits, each of which bind an iron-porphyrin group commonly called heme. Heme binds and releases oxygen or carbon dioxide in response to slight changes in local gas tension. Hemoglobin values are associated with a variety of conditions ranging from anemias (low Hgb), erythrocytosis (high Hgb), thalassemias (aberrant chain synthesis), and sickling disorders (abnormal complex shape).