• Catalog Number K034-H
  • Assay Type Detection Kit
  • Sample Types Urine, Buffer, Tissue Culture Media
  • Sensitivity 1.83 µM
  • Species All Species
  • Assay Duration 15 Minutes
  • Samples/Plate 41 in Duplicate
  • Readout Colorimetric, 560 nm


Assay Principle:

The DetectX® Hydrogen Peroxide (H2O2) Colorimetric Detection Kit provides a straightforward method for quantitatively measuring H2O2 levels in urine, buffer, and tissue culture media. This colorimetric detection kit, with a run time of 15 minutes, is designed for efficient and accurate analysis. Read the complete kit insert thoroughly before conducting the assay. The kit includes a Hydrogen Peroxide standard to establish an accurate standard curve.

Protocol Summary:

  • Introduce standards or diluted samples into the provided transparent microtiter plate.
  • Add Substrate and Horseradish Peroxidase Concentrate to each well, ensuring proper mixing.
  • Incubate the mixture at room temperature for 15 minutes. The color-generating reaction occurs between the Substrate, Horseradish Peroxidase Concentrate, and Hydrogen Peroxide in the sample.
  • After incubation, use a plate reader to detect the generated signal at 560nm. Calculate H2O2 concentration using the intensity and the standard curve.


Hydrogen peroxide (H2O2) is a critical reactive oxygen species in biological systems, primarily produced through the incomplete reduction of oxygen (O2) during cellular respiration. This process yields superoxide anion (O2 -·), which is spontaneously or enzymatically converted into H2O2 by superoxide dismutase.

The presence of H2O2 is a common cellular response to a range of extracellular stimuli, including cytokines like TGF-β1, TNF-α, various interleukins, peptide growth factors (PDGF, EGF, VEGF, bFGF), insulin, and agonists of G protein-coupled receptors (GPCR), including angiotensin II, thrombin, and others.

The Fenton reaction, first described in 1894, highlights the oxidative potential of H2O2 in biological systems. Both exogenously added and intracellularly produced H2O2 can significantly alter the function of various proteins. This potentially leads to the production of highly reactive species like singlet oxygen and peroxynitrite, particularly in reactions involving iron.

Measuring H2O2 in urine, buffer, and tissue culture media is essential for evaluating oxidative stress and its impact on systemic metabolism, biochemical processes, and cellular responses. The DetectX® Hydrogen Peroxide (H2O2) Colorimetric Detection Kit is an important tool for researchers studying oxidative stress, cellular signaling, and the effects of reactive oxygen species in various biological contexts.


Hydrogen Peroxide (H2O2) Colorimetric Detection Kit


In Stock

The DetectX® Hydrogen Peroxide (H2O2) Colorimetric Detection Kit is designed to quantitatively measure H2O2 in a variety of samples.

SKU: K034-H1 Category:

Research Areas