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Specifications

  • Assay Type Detection Kit
  • Sample Types Serum and Plasma (EDTA and Heparin)
  • Sensitivity 0.081 mg/dL
  • Species Human, Mouse, Rabbit, Rat, Sheep
  • Assay Duration 30 Minutes
  • Samples/Plate 43 in Duplicate
  • Readout Colorimetric, 490 nm
  • Standard Curve
  • Description

    Assay Principle: 

    The Serum Creatinine Detection Kit quantitatively measures Creatinine levels in serum and plasma (EDTA and Heparin). The Serum Creatinine Detection Kit is a Detection Kit with a run time of 30 minutes. Please read the complete kit insert for more information before performing this assay.

    Use our provided Creatinine Standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate. Add the DetectX® Creatinine Reagent to each well, tapping the plate to ensure sufficient mixing of reagents. After this addition incubate the mixture at room temperature for 30 minutes. The color-generating reaction occurs between the DetectX® Creatinine Reagent and the Creatinine in the standard or sample. 

    After the 30-minute incubation, use a plate reader to detect and record the generated signal at 490nm. Use the intensity and the standard curve to calculate the Creatinine concentration in the samples. This assay utilizes a kinetic absorbance method to overcome interference by colored compounds in the sample. 

    Background:

    Creatinine is a metabolite of phosphocreatine (p-creatine), a high-energy phosphate that tissues can utilize to produce ATP. Creatine comes from the diet, or enzymes synthesize Creatinine from the amino acids arginine, glycine, and methionine. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood with excretion by the kidneys. In vivo, this conversion appears irreversible, and in vitro, higher temperatures and lower pH favor this conversion. 

    Measuring serum creatinine is one of the most commonly used indicators of renal function. A rise in blood creatinine levels is observed only with marked damage to functioning nephrons. The creatinine clearance test is a good indicator of kidney function. Individuals can accurately calculate Creatinine clearance by using serum creatinine concentration and considering the variables of sex, age, weight, and race.