Assay Principle 

The Testosterone ELISA Kits – Improved Sensitivity quantitatively measures Testosterone in serum, plasma, fecal extracts, and urine. This competitive ELISA has a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay. Use our provided Testosterone standard to generate a standard curve for the assay.

Protocol Summary

• Introduce standards or diluted samples into the provided transparent microtiter plate, pre-coated with goat anti-rabbit IgG antibody.
• Add Testosterone peroxidase conjugate and Testosterone rabbit polyclonal antibody to initiate the immunological reaction.
• Incubate the mixture covered at room temperature with shaking for 2 hours. The reaction varies inversely with the Testosterone concentration in the sample.
• Post-incubation, wash away the excess conjugate and introduce the TMB substrate, which reacts with the bound conjugate to generate a measurable signal.
• Detect the signal at 450nm using a plate reader and calculate the Testosterone concentration based on the standard curve.

Background

Testosterone (4-Androsten-17β-ol-3-one) is a key anabolic steroid hormone from the androgen group, found in mammals, reptiles, birds, and other vertebrates. In mammals, testosterone is primarily secreted in the testes of males and the ovaries of females, with minor contributions from the adrenal glands.

As the principal male sex hormone, testosterone is crucial for the development of reproductive tissues like the testes and prostate. It also promotes secondary sexual characteristics, including increased muscle and bone mass, and the growth of body hair. Beyond its reproductive roles, testosterone significantly influences glucose homeostasis, lipid metabolism, and the prevention of osteoporosis. Epidemiological studies suggest a direct correlation between plasma testosterone levels and insulin sensitivity, with low testosterone levels being associated with an increased risk of type 2 diabetes. This is notably evident in men undergoing androgen deprivation for prostate carcinoma treatment.

The Improved Sensitivity DetectX® Testosterone ELISA Kit is an important tool for researchers and clinicians in endocrinology, reproductive health, and metabolic studies, offering enhanced  testosterone measurement in various biological samples.