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ELISAs are assays with a need to measure the presence and amount of an antibody to a particular molecule. These circulating antibodies can be present because the individual has been given a ‘foreign’ antigen or are self-generated auto-antibodies. The capture of these circulating antibodies is done by immobilizing antigen onto a solid phase. The captured antibody is then quantified by addition of either a general conjugated isotype antibody or a more specific subtype conjugate. We can carry out all stages of this work including conjugating antigen for antibody isolation, immobilize the antigen to solid phase, formulate isolated antibody for use as a standard in the assay, carry out assay optimizations and complete the validation process.
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