Progesterone ISWE Mini-Kit

Features

  • Multi Species
  • CONSISTENCY - Consistent Assays & Supply
  • ECONOMICAL - Enough for 50 Plates
  • COMPLETE - Primary Antibody, Conjugate & Standard
  • TESTED - Tested in fecal extracts against multiple species
  • RELIABLE - CL425 Monoclonal Antibody

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The ISWE Progesterone Mini-Kit is specifically designed to measure Progesterone present in a variety of samples and species for wildlife conservation. The end user must provide or coat their own anti-mouse IgG secondary antibody coated plates, in addition to providing assay buffer, wash buffer, substrate and stop solution. See Related Products for suitable options available.

NOTE: The 50X Concentrate Mouse CL425 Antibody to Progesterone cannot be directly coated onto plates for EIA use. It is an unpurified ascites fluid diluted in a buffer containing proteins and detergents. The antibody WILL NOT bind to uncoated microtiter plates effectively. The end user must use plates coated with goat or donkey anti-mouse IgG.

Kits Include:

ISWE Progesterone Antibody Concentrate

ISWE Progesterone Conjugate Concentrate

Progesterone Standard

Storage Instructions

All components of this kit should be aliquoted into vials upon first thaw. Avoid repeat freeze-thaw cycles.

Store at -20°C until the expiration date of the kit.

Protocols

The CL425 mouse anti-Progesterone is supplied at a 50X concentration. An aliquot should be brought to room temperature and diluted one part with 49 parts of a suitable assay buffer. If your assay buffer has protein, detergent and preservative in it, the diluted antibody will be stable for greater than 1 month at 4°C. Please see Related Products for a suitable Assay Buffer.

The Progesterone-HRP conjugate is supplied at a 50X concentration. An aliquot should be brought to room temperature and diluted one part with 49 parts of a suitable diluent or assay buffer. If you wish to store the conjugate diluted for greater than 1 month at 4°C you should dilute it in a suitable conjugate diluent. Please see Related Products for a suitable Conjugate Diluent.

The Progesterone standard stock is provided at 320 ng/mL (320,000 pg/mL) in a special storage solution.  The solution contains an organic solvent. Prerinse the pipet tip several times to ensure accurate delivery.

NOTE: The dilution of the ISWE Mini-kit antibody and conjugate are such that when diluted 1:50 in Assay Buffer or Conjugate Diluent and added to an Arbor Assays’ antibody coated plate, using 50 μL of sample or standards, 25 μL of conjugate and 25 μL of antibody in a 2 hour room temperature with shaking incubation, followed by our TMB as substrate, these reagents will generate approximately 1.0 OD at 450 nm. YOUR RESULTS WILL VARY DEPENDING ON A NUMBER OF VARIABLES. You will have to titer the antibody or conjugate to obtain the optimum conditions for your assay system.

ISWE003

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