|Corticosterone—the most common glucocorticoid in rodent animal models of stress—is easily measured using ELISA techniques. However, blood sample collection procedures are inherently stressful, and non-invasive methods such as saliva and fecal samples are often preferred. As each species generates a variety of hormone metabolites in differing concentrations, it is important to validate each assay with the species to ensure that the assay usefully indicates physiology. For HPA-related hormones, the use of an ACTH challenge is often used.
A recent study by Andreas Eleftheriou, Rupert Palme and Rudy Boonstra investigated use of a general and a group-specific ELISA to assess stress levels in rodent models. Using a Corticosterone ELISA kit from Arbor Assays, the authors validated the immunoassays for measuring fecal corticosterone metabolites in a deer mouse model of stress.
Our Corticosterone ELISA and Chemiluminescent Assay Kits are the industry’s gold-standard with over 290 citations and counting in high-impact, peer-reviewed journal articles.
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