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Chronic repeated unpredictable stress induces PTSD in mice… and likely humans, too.

Chronic stress can lead to dysregulation of hypothalamic-pituitary-adrenal (HPA) axis function, and is implicated in diseases like depression and post-traumatic stress disorder (PTSD). The development of treatments for stress-related disorders requires a better understanding of the biological basis of HPA axis dysfunction. A recent study by Algamal, et al. investigated the neuroendocrine and biochemical assessment of HPA axis function in a mouse model of chronic stress by inducing chronic stress in mice through a program of repeated unpredictable stress (RUS) followed by chronic social isolation.

The effect of RUS on the HPA axis function was measured partly through analysis of glucocorticoid levels using DetectX®  Corticosterone and cAMP ELISA kits from Arbor Assays. Stressed mice exhibited disruption in HPA-axis signaling and PTSD behavioral deficits such as exaggerated fear memory recall and anxiety-like behavior even one month after being subjected to RUS. These behaviors are similar to those in humans, suggesting this model is suitable for examining the mechanisms behind fear-related abnormalities in PTSD.


  • Multi-format kit allows for measurement of Corticosterone in as little as 2 μL
  • Validated in serum, plasma, saliva, urine, fecal extracts, and tissue culture media across a variety of species
  • Enough reagents for 37 or 229 samples in duplicate
  • Sensitivity of 20.9 pg/mL in 50 μL format and 17.5 pg/mL in 100 μL format
  • Cited in over 300 publications


  • Measure cAMP in 2.5 hours
  • Validated in lysates, urine, plasma, saliva, and tissue samples across mice, humans, rabbits, and more
  • Enough reagents for 39 or 231 samples in duplicate
  • Sensitive enough to measure < 5 fmol cAMP in a sample
  • Cited in over 80 publications

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