Glucocorticoids like corticosterone guide fundamental processes associated with converting sugar, fat, and protein stores to useable energy, inhibiting swelling and inflammation, and suppressing immune responses following a stress event. Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland in response to stimulation by the adrenocorticotropic hormone. It is an important stress marker in nonhuman mammals.
Competitive immunoassays, RIA and EIA methods, are the typical means for measuring levels of corticosterone in biological matrices. Most RIAs or EIAs require solvent extraction techniques to measure serum or plasma corticosterone levels; however, extraction may be very difficult or impossible with mouse samples due to the large volumes of plasma or serum required for most extraction protocols.
Our Corticosterone EIA (K014-H1 and K014-H5) and CLIA (K014-C1 and K014-C5) kits overcome this serum and plasma volume problem by allowing the direct measurement of corticosterone from as little as 1μL of mouse serum or plasma. Serum or plasma samples are treated with an equal volume of Arbor Assays’ Dissociation Reagent that liberates the corticosterone from any binding proteins. The mixture is then diluted 1:50 or greater with assay buffer and added directly to the well of the provided microtiter plate for analysis by EIA/CLIA without any extractions.
The EIA provides results in 90 minutes and the chemiluminescent immunoassay (CLIA) in 2 hours. The EIA standard range spans 10,000 to 78 pg/mL and the CLIA has a range from 3,000 to 12.3 pg/mL with a measured sensitivity of 6.7 pg/mL. The CLIA allows concentrations of <0.004 μM of corticosterone to be measured. Typical c57bl/6j mouse serum corticosterone levels fall at 0.08 ± 0.01 μM and therefore can be measured in this assay without extraction.
Use Our Validated Corticosterone EIA
- Wide range: 10,000 to 78 pg/mL
- 1 μL serum or plasma sample: for duplicate measurements
- No solvents and no extractions
- Results in 90 minutes
- Validated for multiple sample types: plasma, serum, urine, hair, feathers, fecal extracts, and tissue culture media
- Species independent
- High quality and economical
- All with superior 4°C liquid stability
“The Arbor Assays EIA and CLIA for corticosterone are the best kits I have ever used. They are exquisitely sensitive, with easy to follow instructions, and they work beautifully.” Prof. SL, Univ. of Kentucky
Customer’s Validation Data Using 1 μL Mouse Serum without Extraction
Comparison of the Arbor Assays EIA with a Commercial RIA
Typical Data Comparison of our EIA and CLIA Corticosterone Assays