Arbor Assays’ Newest Oxidative Stress Assay
Lipid peroxidation is one of the most commonly reported indices of oxidative stress and has been implicated as a contributing factor in a range of degenerative diseases, including diabetes1, cardiovascular disease, Parkinson’s disease, Alzheimer’s disease2, and psychiatric disorders, such as schizophrenia3. Lipid peroxidation assays are widely used to assess oxidative damage in disease models and Malondialdehyde (MDA) is one of the most commonly reported biomarkers of lipid peroxidation in research and clinical studies.
The DetectX® Universal TBARS/MDA Universal Colorimetric Assay Kit (K077-H1) provides a simple, fast, and user-friendly protocol to measure the MDA in samples exposed to oxidative stress.
- No boiling water baths
- Cost-effective: 2 Plate Kit – Up to 88 samples in duplicate
- Universal: Works with serum, plasma, urine, cells, food samples, and buffer
- Color-coded sample diluent
- 4°C stable, liquid, ready-to-use reagents
In the assay, MDA and other reactive aldehydes react with TBA to produce the pink color species that absorbs at 530–540 nm. The TBARS test is an easy and cost-effective method and widely used for routine analysis of MDA in clinical, biological, and food research laboratories.
1Pasupathi, P., Chandraseker, V., & Kumar, U. S. (2009). Evaluation of oxidative stress, antioxidant and thyroid hormone status in patients with diabetes mellitus. Journal of Medicine, 10(2), 60–66.
2Adly, A. A. M. (2010). Oxidative stress and disease: An updated re-view. Res. J. Immunololgy 3, 129–145.
3Tsaluchidu, S., M., et al. (2008). Fatty acids and oxidative stress in psychiatric disorders. BMC Psychiatry. 8 (Suppl.), 1–3.