Oxidative Stress and Tuberculosis
...levels of glutathione and further compromise levels of enzymes involved in the synthesis and regulation of glutathione. This reduction in available ROS can make it harder for these individuals to...
News & Blog
Pregnenolone-Progesterone-Allopregnanolone Pathway and Schizophrenia
...prolactin. Prolactin inhibits 5a-reductase, the enzyme that catalyzes the conversion of progesterone to allopregnanolone, suggesting one feedback loop that could be responsible for the loss of allopregnanolone normalization as treatment...
Cortisone
...either enzyme, 11ß-HSD1 is found primarily in the liver where it converts cortisone to cortisol while 11ß-HSD2 is found in tissues such as the kidney where cortisol receptor binding is...
Glutathione is Necessary for Hippocampal Neurons to Maintain Healthy Dendrites and Cognitive Function
...knock down the catalytic subunit of Glutamate-Cysteine Ligase (GCL), which is the rate-limiting enzyme of cellular GSH production. The genetically engineered GCL knocked down mouse (small hairpin RNA against GCL...
Eat Your Vegetables!
...antioxidant enzyme mimetics (AEM) improve endothelial dysfunction and reduce uremia associated cardiovascular risk through potentiation of antioxidant pathways. Flavanoids and the glutathione peroxidase mimetics ebselen and N-acetylcysteine reduced p38MAPK activation,...
Can Raw Cane Sugar Help Reduce Stress?
...marker) was affected in restraint-stressed mice treated with Kokuto extracts. Serum corticosterone was measured using Arbor Assays’ Corticosterone Enzyme Immunoassay Kit, K014-H1/H5. Oral administration of a methanol Kokuto extract suppressed...
Is Glutathione The Ultimate Antioxidant?
...molecule in varying ways including neutralizing reactive oxygen species or maintaining cysteine in reduced forms of protein. GSH can be regenerated from GSSG by the enzyme glutathione reductase. Although over...
Non-Invasive Whale Hormone Measurement
...progestins using an in-house, lab-derived radioimmunoassay (RIA). Testosterone was screened using two assays; the first assay was an in-house RIA and the second was a commercial colorimetric enzyme immunoassay (Testosterone...
What More Could You Ask For?
Nitric oxide (NO) is a diffusible, transient, reactive molecule, and an important regulator of cardiovascular, neurological, and immunological systems. NO is generated endogenously by constitutive or induced enzymes like Nitric...
3′,3′-Cyclic GAMP Assay: The Newest Tool in the Researcher’s Arsenal
...mediator of bacterial signal transduction and regulation, controlling a range of diverse targets including transcription, enzyme activity and cell cycle progression. 3’3’-cGAMP signaling in bacteria is regulated in-part by gene...
Inhibiting Doxorubicin-Induced Cardiotoxicity
...damage by improving antioxidant enzymes activities in cardiomyocytes. Catalase activity was measured using Arbor Assays’ kit, K033-H1, and we also produce kits for measuring superoxide dismutase activity, K028-H1, and the...
Keeping Your Elephants Happy
...by developing highly sensitive and specific non-radioactive enzyme immunoassays (EIAs) using Arbor Assays’ suite of custom assay development reagents. These reagents are made available as part of a long-standing collaboration...
Saving the Kea
...and measured the concentrations using a corticosterone enzyme immunoassay. This protocol was established using 96-well plates, pre-coated with Arbor Assays goat-anti-rabbit IgG antibody (catalog number A009). Following validation, the immunoassay...
Bromance at the zoo: Male gorillas benefit from increased oxytocin in bachelor grouped housing
...managed WLG were kept in their own enclosure to prevent physical altercations but with visual, auditory, and olfactory access to other gorillas. Hormone levels from urine were measured using enzyme...