Cell Signaling
...lead to the activation of a variety of enzymes, proteins, and ion channels. One of the most prominent enzymes activated by cAMP is PKA. Once activated PKA in turn can...
News & Blog
A Healthy Prebiotic Sugar from Seaweed
...humans don’t possess enzymes that breakdown XOS, they are unable to be digested and pass through the gastrointestinal tract. The prebiotic nature of XOS was recently discovered and evidence suggests...
Sensitive Cyclic Nucleotide Assays
...measurement of cyclic nucleotides. CYCLIC AMP DIRECT CHEMILUMINESCENT ELISA KITS (K019-C1/C5) CYCLIC AMP DIRECT ELISA KITS (K019-H1/H5) CYCLIC GMP DIRECT ELISA KITS – IMPROVED SENSITIVITY (K065-H1/H5) ACTIVATORS & INHIBITORS IBMX...
Sensitive Assays for Cyclic Nucleotide Signaling
...KITS (K019-H1/H5) CYCLIC GMP DIRECT ELISA KITS – IMPROVED SENSITIVITY (K065-H1/H5) ACTIVATORS & INHIBITORS IBMX (P019-100MG/1GM): Increases cellular cAMP and cGMP levels, activating cyclic-nucleotide-regulated protein kinases. DMXAA (P024-5MG/25MG): STING agonist selective for mouse...
Role of Rcan1 in Vascular Contractility and Hypertension
Interaction between the serine/threonine protein phosphatase calcineurin and the NFAT family of transcription factors plays a critical regulatory role in the regulation of a variety of physiological functions. Calcineurin inhibitors...
Can PGE2 Help Repair A Broken Spine?
...PGE2 synthesis. The Arbor Assays DetectX® PGE2 Enzyme Immunoassay (ELISA), K051-H1/H5, was used to quantify PGE2 level. Further western blotting, immunohistochemistry, qPCR, and ELISA experiments were performed to identify and...
Sensitive Assays for Cyclic Nucleotide Signaling
Cyclic adenosine monophosphate (cyclic AMP or cAMP) and cyclic guanosine monophosphate (cyclic GMP or cGMP) are prolific intracellular signaling molecules. cAMP is synthesized from adenosine triphosphate (ATP) by the enzyme...
DHEA-S Measurements Made Simple
...enzymes. DHEA may then be reversibly converted into a more stable sulfate ester, dehydroepiandrosterone sulfate (DHEA-S) by the enzyme DHEA sulfotransferase. DHEA-S can be converted back into DHEA by steroid...
FRAP – Measuring antioxidant potential
...a complex system of specific enzymes and non-enzyme antioxidant compounds. The capacity of a system to reduce or break down reactive oxygen species can be viewed as an indication of...
Time of Day Influences Inflammatory Response to Cytotoxic Chemotherapy
...of enzymes involved in Cyclo/DOX metabolism were examined in the liver samples to determine if time of injection may influence drug metabolism. Chemotherapy significantly increased expression of Cyp2b10, Cyp3a13 and...
Suppressing Neutrophils in Inflammatory Response
...been shown to either directly activate PKA or to inhibit phosphodiesterase 4 (PDE4), the primary enzyme responsible for cAMP hydrolysis in neutrophils and other immune cells. Isolating the active compounds...
Dopamine D1 Receptor Signaling for Steroidogenic Differentiation
...molecule inducer of 3β-hydroxysteroid dehydrogenase, an essential enzyme for steroidogenesis in adrenogonadal cells. Cabergoline is a dopamine D2 receptor agonist that also has low-affinity activity on dopamine D1 receptors. Further...
DHEA-S Measurements Made Simple
...enzymes. DHEA may then be reversibly converted into a more stable sulfate ester, dehydroepiandrosterone sulfate (DHEA-S) by the enzyme DHEA sulfotransferase. DHEA-S can be converted back into DHEA by steroid...
Immune-Mediated Arthritis and the HPA Axis
...are associated with abnormal HPA responses. What causes this abnormal HPA axis response is not currently understood. A recent paper by Sattler et al. focused on the enzyme 11β-hydroxysteroid dehydrogenase...