Search Results For "enzyme inhibitors and activators"

Super Sensitive Cell Signaling Assays

SENSITIVE ASSAYS FOR CYCLIC NUCLEOTIDE SIGNALING

Cyclic adenosine monophosphate (cyclic AMP or cAMP) and cyclic guanosine monophosphate (cyclic GMP or cGMP) are prolific intracellular signaling molecules. The critical role of cyclic dinucleotides continues to emerge. In mammalian cells, cGAMP is implicated in innate immune response to foreign or harmful DNA.… » Read More


Cyclic AMP Direct Chemiluminescent ELISA Kit

The DetectX® Cyclic AMP (cAMP) Chemiluminescent ELISA Kits are designed to quantitatively measure cAMP present in cell lysates, plasma, urine, saliva, tissue and culture media samples. The supplied Sample Diluent will lyse cells, stabilize cAMP and stop phosphodiesterase activity. A cAMP standard is provided to generate a standard curve for the assay.… » Read More


Cortisone ELISA Kit

The DetectX® Cortisone Enzyme Immunoassay Kit is designed to quantitatively measure cortisone present in extracted dried fecal samples, urine, saliva, plasma, and serum samples. This kit measures total cortisone in serum and plasma and in extracted fecal samples. A cortisone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve.… » Read More


Triiodothyronine (T3) ELISA Kit

The DetectX® Triiodothyronine (T3) Enzyme Immunoassay kit is designed to quantitatively measure T3 present in urine, extracted serum and plasma, extracted dried fecal samples, and tissue culture media samples. This kit measures total T3 in extracted serum and plasma and in extracted fecal samples.… » Read More


Aldosterone ELISA Kit

The DetectX® Aldosterone Enzyme Immunoassay kit is designed to quantitatively measure Aldosterone present in extracted serum and plasma, or in urine, extracted dried fecal samples, and tissue culture media samples. An aldosterone stock solution is provided to generate a standard curve.… » Read More


Apicidin

Potent inhibitor of histone deacetylase (HDAC) activity and proliferation. Induces cell cycle arrest at the G1 phase. At 100 nM it induces a long lasting hyperacetylation of histone H4 while that induced by trichostatin is transient. Stimulates apoptosis. Apoptosis is induced via induction of Fas/Fas ligand.… » Read More


Protein Kinase A (PKA) Colorimetric Activity Kit

The newly improved DetectX® Protein Kinase A Colorimetric Activity Kit provides a sensitive and reliable method for screening inhibitors or activators of PKA and for quantitating PKA activity in a wide range of sample types. The 96-well format kit utilizes a synthetic peptide as a substrate for PKA and a rabbit monoclonal antibody that recognizes the phosphorylated form of the substrate with a three hour assay.… » Read More


Sensitive Cyclic Nucleotide Assays

SENSITIVE ASSAYS FOR CYCLIC NUCLEOTIDE SIGNALING

Cyclic adenosine monophosphate (cyclic AMP or cAMP) and cyclic guanosine monophosphate (cyclic GMP or cGMP) are prolific intracellular signaling molecules. The critical role of cyclic dinucleotides continues to emerge. In mammalian cells, cGAMP is implicated in innate immune response to foreign or harmful DNA.… » Read More


DMXAA

DMXAA is a STING (Stimulator of Interferon Genes) agonist selective for mouse STING. Intratumoral administration of DMXAA resulted in tumor regression and complete rejection in mouse xenografts. Tumor regression induced by DMXAA results from a cascade of cellular events which include disruption of tumor vasculature followed by the release of chemokines which trigger the recruitment of immune cells.… » Read More


H-151

H-151 (941987-60-6) is a potent, irreversible inhibitor of Stimulator of Interferon Genes (STING) in mouse and human cells. H-151 covalently binds to Cys91 of STING preventing activation via blockade of palmitoylation and clustering STING. H-151 blocks STING induced expression of pro-inflammatory cytokines and reduces inflammation in models of auto-inflammatory disease.… » Read More


BRL-50481

BRL-50481 is a potent and selective inhibitor of phosphodiesterase-7 (PDE7), Ki=180 nM. Induces apoptosis in chronic lymphocytic leukemia cells which express PDE7B. Increases mineralization in osteoblasts differentiated from hMSC. Cell permeable.

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Sodium Valproate

An anticonvulsant used in the treatment of epilepsy, anorexia nervosa, panic attack, anxiety disorder, post traumatic stress disorder, migraine and bipolar disorder. Valproate is also a histone deacetylase inhibitor (IC50 = 400 uM) that exhibits anticancer, anti-inflammatory and neuroprotective effects. Displays anticonvulsive activity via an increase in GABA levels and decreases Abeta production in animal models of Alzheimer’s disease.… » Read More


Cyclic AMP Direct ELISA Kit

The DetectX® Cyclic AMP (cAMP) Direct Immunoassay kit is designed to quantitatively measure cAMP present in lysed cells, tissue, plasma, urine, saliva and culture media samples. The kit is unique in that all samples and standards are diluted into an acidic Sample Diluent which is designed to lyse cells, inactivate PDE enzymes, and stabilize cAMP.… » Read More


Thyroxine (T4) ELISA Kit

The DetectX® Thyroxine (T4) ELISA Kit is designed to quantitatively measure T4 present in serum, EDTA plasma, tissue culture media samples and fecal extracts.  A thyroxine standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve.… » Read More


Goat anti-Rabbit IgG

An affinity purified Goat Antibody to Rabbit IgG, Fc region. Supplied in PBS with azide as preservative. Affinity purified on a column with covalently coupled rabbit IgG, Fc attached.

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Donkey anti-Sheep IgG

An affinity purified Donkey Antibody to Sheep IgG,(H&L). Supplied in PBS with azide as preservative. Affinity purified on a column with covalently coupled Sheep IgG attached.

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ST2 Human ELISA Kit

The DetectX® human ST2 ELISA Kit is designed to quantitatively measure ST2 present in a variety of samples and tissue culture media. A recombinant human ST2 standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture ST2. … » Read More


Goat anti-Mouse IgG

An affinity purified Goat Antibody to Mouse IgG, Fc region. Supplied in PBS with azide as preservative. Affinity purified on a column with covalently coupled mouse IgG, Fc attached.

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Histone Demethylase (HDM) Fluorescent Activity Kit

The DetectX® Universal Histone Demethylase (HDM) Fluorescent Activity Kit allows all known HDMs to be measured in low, medium and high throughput fashion. Run the demethylase reaction and after completion simply add the supplied Formaldehyde Detection Reagent to each well and read the fluorescent signal generated.… » Read More


Butyrylcholinesterase Fluorescent Activity Kit

The DetectX® Butyrylcholinesterase (BChE) Fluorescent Activity kit is a simple, sensitive and stable kit that measures BChE activity in a wide variety of samples. The kit contains everything needed to measure activity, including the microtiter plate. Diluted samples are added to the wells.… » Read More


Zebrafish Id1 Rbt pAb, Protein A purified

The Id proteins (Inhibitors of DNA Binding/Differentiation) represent a family of dominant negative regulators of the basic helix-loop-helix transcription factors whose activities result in delayed cell differentiation and prolonged proliferation. The down-regulation of Id1 is necessary for differentiation to proceed in many cell lineages.… » Read More


Acetylcholinesterase Fluorescent Activity Kit

The DetectX® Acetylcholinesterase (AChE) Activity kit is designed to quantitatively measure AChE activity in a variety of samples. A human AChE standard is provided to generate a standard curve for the assay. The kit utilizes a proprietary non-fluorescent molecule, ThioStar®, that covalently binds to the thiol product of the reaction between the AChE Substrate and AChE in the standards or samples, yielding a fluorescent product read at 510 nm in a fluorescent plate reader with excitation at 390 nm.… » Read More


Post-Stroke Dementia Biomarkers

Stroke is the fifth leading cause of death in the United States and is a major cause of serious disability for adults. Post-stroke dementia (PSD) is the major form of dementia in the elderly. Of the 800,000 people in the US that have strokes every year, about 10% of first-time stroke victims will suffer from PSD, while 30% of recurrent stroke patients suffer from this type of dementia.… » Read More


Keeping Your Elephants Happy

KEEPING YOUR ELEPHANTS HAPPY

A new longitudinal study from ISWE members.

A basic understanding of wildlife endocrinology is critical to ensure the health and welfare of endangered species. Hormone analysis is a widely accepted tool for monitoring reproductive function and responses to stressors in captive and free-ranging animals. Understanding… » Read More


17-Hydroxyprogesterone ELISA Kit

The DetectX® 17-Hydroxyprogesterone Enzyme Immunoassay kit is designed to quantitatively measure 17-Hydroxyprogesterone present in extracted serum and plasma, or in urine, extracted dried fecal samples, and tissue culture media samples. A 17-Hydroxyprogesterone stock solution is provided to generate a standard curve.… » Read More


Cortisone Chemiluminescent ELISA Kit

The DetectX® Cortisone Chemiluminescent Immunoassay (CLIA) kit is designed to quantitatively measure Cortisone present in samples. A cortisone standard is provided to generate a standard curve for the assay. After a two hour incubation the plate is washed and the chemiluminescent substrate is added.… » Read More


Cortisol ELISA Kit

The DetectX® Cortisol Enzyme Immunoassay Kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and culture media samples. This kit measures total cortisol in extracted samples, serum and plasma and free cortisol in saliva and urine.… » Read More



Catalase Colorimetric Activity Kit

The DetectX® Catalase Activity Kit is designed to quantitatively measure catalase activity in a variety of samples. A bovine catalase standard is provided to generate a standard curve for the assay. Samples are diluted in the provided Assay Buffer and added to the wells of a half area plate.… » Read More


3′,3′-Cyclic GAMP Assay: The Newest Tool in the Researcher’s Arsenal

Cyclic dinucleotides are the most abundant second messengers in bacteria, modulating a variety of cellular activities in prokaryotic cells.  3’3’-cyclic GAMP (cyclic [G(3’,5’)pA(3’,5’)p], previously known as cGAMP) is a key mediator of bacterial signal transduction and regulation, controlling a range of diverse targets including transcription, enzyme activity and cell cycle progression. … » Read More


Oxidative Stress, Copper, and Glutathione

Regulation of Copper Transport and Cell Viability During Copper Metabolism

Copper (Cu) is an essential cofactor in virtually all organisms, and tight control of Cu metabolic pathways are critical for optimal cellular viability.  Cu is required for proper function of a variety of important enzymes, such as cytochrome c oxidase, ceruloplasmin, and Cu/Zn superoxide dismutase.… » Read More


20-Hydroxyecdysone ELISA Kit

The first insect molting hormone, which is also known as Ecdysone was isolated from a silkworm pupae in 1954. Later in 1996, 20-Hydroxyecdysone was identified and recognized as a derivative of ecdysone. Initially, the name ecdysone was given to these compounds since it was known to regulate the decay of old skin/shell in arthropods.… » Read More


ThioStar® Thiol Detection System

The ThioStar® Thiol Detection Reagent allows users to accurately determine the extent of free thiol content in samples. ThioStar is converted to a brightly fluorescent product upon reaction with the thiol in the sample. The thiol in the sample can either be one that is generated by a reaction, such as the end product of an enzymatic reaction such as glutathione, or can be the cysteine content of the protein to be measured.… » Read More



Nitric Oxide (NO) Colorimetric Detection Kit

The DetectX® Nitric Oxide (NO) Detection Kit is designed to quantitatively measure Nitrate and Nitrite present in a variety of samples. Nitric Oxide content is derived from the sum of Nitrate and Nitrite. Both Nitrate and Nitrite standards are provided to generate standard curves for the assay and all samples should be read off the appropriate standard curve.… » Read More


Glutathione Reductase (GR) Fluorescent Activity Kit

The DetectX® Glutathione Reductase (GR) Fluorescent Activity Kit determines GR activity by the amount of GSH generated from the reduction of GSSG with a non-fluorescent molecule, ThioStar®, that covalently binds the free thiol group on GSH to yield a highly fluorescent product.… » Read More


Glutathione (GSH) Fluorescent Detection Kit (384-Well Plate)

The DetectX® Glutathione (GSH) Fluorescent Detection Kit utilizes a proprietary non-fluorescent molecule, ThioStar®, that covalently binds to the free thiol group on GSH to yield a highly fluorescent product. After mixing the sample or standard with ThioStar® and incubating at room temperature for 15 minutes.… » Read More


Glutathione (GSH) Colorimetric Detection Kit

The DetectX® Glutathione (GSH) Colorimetric Detection Kit utilizes a colorimetric substrate that reacts with the free thiol group on GSH to yield a highly colored product. All reagents are in solution and require simple dilution for use in the assay.… » Read More


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Our philosophy at Arbor Assays is to build the highest quality detection and immunoassay products for clinically important biomolecules. We build robust immunoassay and enzyme activity kits to quantitate biomolecules in biological matrices.

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Cyclic GMP Direct Chemiluminescent ELISA Kit

The DetectX® Direct Cyclic GMP (cGMP) Chemiluminescent Immunoassay (CLIA) kit will measure cGMP present in lysed cells, EDTA plasma, urine, saliva and culture media samples. The kit is unique in that all samples and standards are diluted into an acidic Sample Diluent, which contains special additives and stabilizers, for cGMP measurement.… » Read More


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