The neuropeptides oxytocin and vasopressin were isolated and synthesized by Vincent du Vigneaud at Cornell Medical College in 1953, work for which he received the Nobel Prize in Chemistry in 1955. Oxytocin is highly conserved across vertebrate species and consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. It is produced primarily in the hypothalamus and released from the posterior pituitary into systemic circulation. Oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 and/or 8. For example in mesotocin, a common peptide found in certain fishes, reptiles, birds, amphibians, marsupials and non-mammalian tetrapods, the leucine at residue 8 of oxytocin is substituted for isoleucine.
The classical actions of oxytocin are stimulation of uterine smooth muscle contraction during labor and milk ejection during lactation, however oxytocin also plays an important role in many other reproduction-related functions in both males and females. Oxytocin, either systemic or synthesized in peripheral tissues, has been implicated in behavioral actions such as orgasm, social recognition, pair bonding, anxiety, and maternal behaviors.
The Arbor Assays’ Oxytocin Chemiluminescent Immunoassay (K048-C1/C5), breaks the 10 pg/mL barrier, and along with the Oxytocin Enzyme Immunoassay (K048-H1/H5), offers reproducible, reliable, affordable, consistent and quantitative detection of oxytocin, mesotocin, and isotocin. After an overnight incubation at 4°C, the plate is washed and substrate is added. For the CLIA kits, the substrate reacts with the bound oxytocin-peroxidase conjugate to produce light. For the EIA kits, the TMB substrate reacts with the bound oxytocin-peroxidase conjugate to produce a yellow colored product read at 450 nm. An oxytocin standard is provided to generate a standard curve for the assays. Both kits are designed to measure oxytocin or oxytocin-like peptides present in serum, plasma, clarified milk and tissue culture media samples from a wide range of species including mammals, fish, reptiles, birds and marsupials.
Oxytocin EIA and CLIA Standard Curve
The Oxytocin EIA kit has been shown to give parallel response with extracted gorilla saliva samples. The data, presented below, was generated at the Cleveland Metroparks Zoo.
|Aug 16 2016||
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