Arbor Assays

What’s in a Whisker?

WHAT’S IN A SEAL’S WHISKER?

WHY?

Scientists are always looking for unique ways to obtain non-invasive tissues for physiological analysis of animals. Most body tissues or fluids such as blood, urine or fecal extracts provide only a glimpse of the reproductive and stress conditions at a given time. In a recent study, Keogh et al. (2021) wanted to determine if whiskers can provide a longitudinal record of reproductive and stress conditions throughout an animal’s lifetime.

METHOD

If hormones are absorbed into whiskers over time, they should provide a longitudinal record of reproductive state and physiological correlates of stress. To test this hypothesis, whiskers from female Steller sea lions (Eumetopias jubatus) and northern fur seals (Callorhinus ursinus) were sectioned, serially cut into pieces and pulverized to extract steroid hormones. Whisker extracts were assayed for four hormones by using Arbor Assays Cortisol (K003-H), Progesterone (K025-H), Testosterone (K032-H) and 17β-estradiol (K030-H / KB30-H) kits to examine the effects of age, sex, class or the reproductive status over time.

FINDINGS

Results showed that all hormones were measurable throughout the length of a whisker, providing multiple years of hormone levels. Reproductive cyclical patterns were observed from whiskers for progesterone, providing evidence of previous pregnancies or luteal stages. Progesterone and testosterone levels from whiskers were also helpful in determining the sex of unknown individuals. Cortisol levels were higher near the root of the whisker and declined towards the end of the whisker, without showing a meaningful pattern. All in all, this study highlights a potential use of whiskers to track multi-year physiological parameters in sea-lions and seals, revealing another cool feature about whiskers.

LEARN MORE ABOUT THE SENSITIVE, HIGHLY-CITED DETECTX® KITS USED IN THIS STUDY:

 

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