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MPO (Human) EIA Kit

Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role.  Normally stored in azurophilic granules, MPO is released into the extracellular space during degranulation. There, as part of the neutrophils “respiratory burst” it produces hypochlorous acid from hydrogen peroxide and Cl. MPO also uses hydrogen peroxide to oxidize tyrosine to the tyrosyl radical. Both hypochlorus acid and tyrosyl are cytotoxic and when present can kill bacteria and other pathogens. Hereditary deficiency of myeloperoxidase predisposes individuals to immune deficiency.

Studies have shown an association between elevated MPO levels and coronary artery disease1, and in 2003 it was suggested that MPO may serve as a sensitive predictor of myocardial infarction in patients complaining of chest pain2.  Since that time the clinical utility of MPO testing in cardiac patients has been solidly established in the literature with well over 100 papers published. In 2010 Heslop et al. further refined the clinical application by determining that measuring both MPO and C-reactive protein provided more accurate prediction of mortality risk then measuring just CRP alone.3

Arbor Assays has partnered with Athens Research and Technology to offer the best MPO EIA kit available. With 30 years of experience purifying highly active native human proteins, Athens Research and Technology provided superior quality human MPO, which the Arbor Assays team then developed into a top of the line EIA featuring a unique antibody pairing along with careful optimization and our trademark exceptional quality control. The Athens Research and Technology/Arbor Assays human MPO EIA kit has been validated for serum, platelet-poor heparin plasma, saliva, urine and tissue culture media with results in just 2.5 hours and a sensitivity of 0.68 ng/ml. The kit is available for purchase from either company (Arbor Assays cat.# K060-H1, Athens Research and Technology cat.# K060-H1)

Human Myeloperoxidase EIA Kit Features

-High purity native human MPO standard
-Quick Results – 2.5 hours
-Sensitivity – 0.068 ng/ml
-Samples / Kit – 38 samples in duplicate
-Stability – liquid reagents stable at 4°C

References

  1. Zhang et al. (2001) Association between myeloperoxidase levels and risk of coronary artery disease; JAMA, 286 2135
  2. Brennan et al. (2003) Prognostic value of myeloperoxidase in patients with chest pain; New Engl. J. Med., 349 1595
  3. Heslop et al. (2010) Myeloperoxidase and C-reactive protein have combined utility for long-term prediction of cardiovascular mortality after coronary angiography; J. Am. Coll. Of Cardiol. 55 1102

Related Products:

Arbor Assays Kits:

Allopregnanolone EIA & CLIA – K044-H & K044-C
Atrial Natriuretic Peptide (ANP) EIA – K026-H
Endothelin (ET-1) EIA Kit – K045-H1
Nitric Oxide (NO) Colorimetric Detection Kit – K023-H1
Hemoglobin Colorimetric Detection Kit – K013-H1
Prostaglandin E2 (PGE2) Multi-Format Kits – K051-H
Protein Kinase A (PKA) Activity Kit – K027-H1
ST2 Human EIA Kit – K055-H1

Athens Research and Technology Reagents:

Myeloperoxidase, Human Neutrophils – 16-14-130000
High Density Lipoprotein (HDL), Human, Plasma – 12-16-080412
Ceruloplasmin, Human Plasma – 16-16-030518
Superoxide Dismutase (SOD), Human Erythrocytes – 16-05-191504
Elastase, Human Neutrophils – 16-14-051200
Eosinophil Peroxidase (EPO), Human Eosinophils – 16-15-160518
Lactoferrin, Human Milk – 16-13-120103

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Arbor Assays will be closed Wednesday July 4th through Friday July 6th for the Independence Day Holiday